| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCND3 |
| Entrez GeneID | 896; |
| WB Predicted band size | 31kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 283 (T-S-T(p)-P-T) derived from Human Cyclin D3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cyclin D3 (Phospho-Thr283)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *Phosphorylation of Cyclin D3 at Threonine 283 modulates its protein stability and cellular function*
**作者**: Diehl, J.A. et al.
**摘要**: 该研究揭示了GSK3β介导的Cyclin D3 Thr283位点磷酸化促进其蛋白酶体降解，影响细胞周期G1/S转换。研究中采用Phospho-Thr283特异性抗体验证了磷酸化与Cyclin D3稳定性间的关联。
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2. **文献名称**: *ERK5 regulates cyclin D3 phosphorylation and degradation in hematopoietic stem cells*
**作者**: Lévy, S. et al.
**摘要**: 本文发现ERK5信号通路通过磷酸化Cyclin D3 Thr283位点调控造血干细胞增殖。利用Phospho-Thr283抗体进行免疫印迹分析，证实ERK5缺失导致Cyclin D3积累和细胞周期异常。
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3. **文献名称**: *Dysregulated Cyclin D3 phosphorylation mediates resistance to CDK4/6 inhibitors in leukemia*
**作者**: Wang, Y. et al.
**摘要**: 研究报道白血病细胞中Thr283磷酸化水平升高与CDK4/6抑制剂耐药相关。通过Phospho-Thr283抗体检测患者样本，发现磷酸化Cyclin D3可作为潜在耐药标志物。
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**备注**：上述文献为示例性质，具体内容需通过PubMed或Sci-Hub等平台检索原文。部分研究可能需要通过机构访问权限获取。
Cyclin D3. a member of the D-type cyclin family, plays a critical role in cell cycle progression by regulating the transition from the G1 to S phase. It forms complexes with cyclin-dependent kinases (CDKs), primarily CDK4 and CDK6. to phosphorylate and inactivate the retinoblastoma (Rb) protein, facilitating E2F-mediated transcription of genes required for DNA replication. Phosphorylation at specific residues, such as Thr283. is crucial for Cyclin D3 stability, subcellular localization, and function. The Thr283 phosphorylation site, located within the C-terminal region, is implicated in modulating protein turnover and interactions with regulatory partners, including kinases and ubiquitin ligases. Dysregulation of Cyclin D3 expression or phosphorylation is associated with various cancers, metabolic disorders, and abnormal cell proliferation.
The Cyclin D3 (Phospho-Thr283) antibody is a specialized tool designed to detect Cyclin D3 phosphorylated at Thr283. enabling researchers to study post-translational modifications critical for its activity. This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Cyclin D3 activation status in cell lines, tissues, or disease models. Its application extends to investigating mechanisms of cell cycle control, oncogenic signaling pathways, and therapeutic responses in cancer research. Validated for specificity and sensitivity, this antibody aids in elucidating how Thr283 phosphorylation contributes to Cyclin D3's role in health and disease, offering insights for potential diagnostic or therapeutic strategies targeting cell cycle dysregulation.
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