| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HHF5, CD220 |
| Entrez GeneID | 3643; |
| WB Predicted band size | 95kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 1361 (I-P-Y(p)-T-H) derived from Human IR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IR (Phospho-Tyr1361)抗体的参考文献示例(注：以下内容为模拟示例，具体文献需根据实际检索补充)：
1. **文献名称**：*"Insulin-induced phosphorylation of Tyr1361 regulates IRS-1 interaction and downstream signaling"*
**作者**：Zhang et al.
**摘要**：研究通过Phospho-Tyr1361抗体验证了胰岛素刺激后IR在Tyr1361位点的磷酸化，并证明该位点对IRS-1结合及PI3K/AKT信号激活至关重要。
2. **文献名称**：*"Dysregulation of IR tyrosine phosphorylation in type 2 diabetes models"*
**作者**：Kimura et al.
**摘要**：利用Phospho-Tyr1361抗体进行Western blot分析，发现糖尿病小鼠肝脏组织中Tyr1361磷酸化水平显著降低，提示其与胰岛素抵抗的相关性。
3. **文献名称**：*"Structural basis of IR activation by phosphorylation at Tyr1361"*
**作者**：White et al.
**摘要**：通过该抗体的免疫沉淀实验结合质谱分析，揭示了Tyr1361磷酸化诱导的IR构象变化及其对激酶活性调控的分子机制。
4. **文献名称**：*"Role of Tyr1361 phosphorylation in cancer-associated insulin receptor signaling"*
**作者**：Chen et al.
**摘要**：研究使用Phospho-Tyr1361抗体进行免疫荧光染色，发现肿瘤细胞中该位点异常磷酸化与PI3K/mTOR通路的过度激活相关。
(注：以上文献为示例性质，实际引用需根据具体研究补充真实来源。)
The insulin receptor (IR) is a transmembrane tyrosine kinase receptor critical for regulating glucose homeostasis, cell growth, and metabolism. Upon insulin binding, IR undergoes autophosphorylation at specific tyrosine residues, including Tyr1361. located in the kinase activation loop. Phosphorylation at Tyr1361 (pY1361) is essential for full kinase activation and downstream signaling through pathways like PI3K/Akt and MAPK, which mediate insulin's metabolic and mitogenic effects.
The IR (Phospho-Tyr1361) antibody is a selective tool designed to detect IR when phosphorylated at Tyr1361. It is widely used in research to study insulin receptor activation dynamics, insulin resistance mechanisms, and dysregulated signaling in conditions like type 2 diabetes, obesity, and cancer. This antibody is typically validated in applications such as Western blotting, immunoprecipitation, and immunohistochemistry, often using cell lysates or tissues from insulin-stimulated models.
Importantly, phosphorylation at Tyr1361 is transient and tightly regulated, making this antibody valuable for capturing activation states. Researchers also use it to explore therapeutic interventions targeting IR signaling or to assess biomarkers in metabolic disorders. Specificity is ensured by testing in knockout models or with phosphatase-treated samples. Its role in distinguishing activated IR from other tyrosine-phosphorylated proteins underscores its utility in both basic and translational research.
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