| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | S6K, PS6K, S6K1, STK14A, p70-S6K |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around serine 371 (V-D-S(p)-P-D) derived from Human p70 S6 Kinase. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p70S6 Kinase (Phospho-Ser371)抗体的3篇代表性文献的模拟示例(非真实文献，仅供格式参考)：
1. **文献名称**: *"mTOR-dependent phosphorylation of p70S6K at Ser371 regulates its activity in mammalian cells"*
**作者**: Pearce, L.R. et al.
**摘要**: 研究揭示mTORC1通过磷酸化p70S6K的Ser371位点调控其激酶活性，并开发了特异性识别该位点的抗体，验证其在细胞增殖中的作用。
2. **文献名称**: *"Phosphorylation of Ser371 within the autoinhibitory domain of p70S6K suppresses its kinase activity"*
**作者**: Magnuson, B. et al.
**摘要**: 利用Phospho-Ser371抗体证明，该位点的磷酸化通过抑制p70S6K的C端自抑制结构域，增强其对下游底物(如S6蛋白)的活性。
3. **文献名称**: *"A novel antibody-based assay for detecting p70S6K activation in human tumor samples"*
**作者**: Zhou, X. et al.
**摘要**: 开发了一种基于Phospho-Ser371抗体的免疫组化方法，用于检测癌症组织中p70S6K的激活状态，并发现其与mTOR抑制剂治疗响应相关。
**备注**：以上文献为示例，实际引用请通过PubMed或学术数据库查询最新研究。
The p70 S6 kinase (p70S6K), a serine/threonine kinase belonging to the AGC kinase family, plays a critical role in the mTOR (mechanistic target of rapamycin) signaling pathway, regulating cell growth, proliferation, and survival by modulating protein synthesis. Phosphorylation at Ser371 (p-Ser371) is a key regulatory event in p70S6K activation. This phosphorylation, mediated by mTOR complex 1 (mTORC1) or other upstream kinases, induces conformational changes that facilitate subsequent phosphorylation at Thr389. a prerequisite for full kinase activity. The p70S6K (Phospho-Ser371) antibody is a specific immunological tool designed to detect this phosphorylation event, enabling researchers to assess mTORC1 pathway activation status in various biological contexts.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study signaling dynamics in response to growth factors, nutrients, or stress conditions. Its applications span cancer research (where mTOR signaling is frequently dysregulated), metabolic studies, and investigations into aging or neurodegenerative diseases. Validation typically includes testing in knockout models or phosphorylation-blocking experiments to ensure specificity. Researchers often pair it with total p70S6K antibodies to quantify activation ratios. Commercial versions are available from multiple suppliers (e.g., CST, Abcam), with distinct host species (rabbit, mouse) and conjugate options. Proper storage (in glycerol-containing buffers at -20°C) and optimized dilution protocols are crucial for maintaining antibody performance.
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