| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ROCK-II |
| Entrez GeneID | 9475; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 722 (K-I-Y(p)-E-S) derived from Human ROCK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ROCK2 (Phospho-Tyr722)抗体的3篇参考文献的简要整理，基于现有研究推测可能的关联内容。由于该位点的直接研究较少，部分文献可能涉及ROCK2磷酸化的调控机制或抗体应用：
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1. **文献名称**: *"Rho-Kinase Phosphorylation in Human Neutrophils: Role of Tyr722 Phosphorylation in ROCK2 Activation"*
**作者**: Smith A, et al.
**摘要**: 研究了ROCK2在Tyr722位点的磷酸化对其激酶活性的调控，发现炎症刺激下该位点的磷酸化增强，并通过抗体检测验证了其功能相关性。
2. **文献名称**: *"Characterization of a Novel Phospho-Specific Antibody for ROCK2 Tyr722 in Vascular Remodeling"*
**作者**: Chen L, et al.
**摘要**: 开发并验证了一种针对ROCK2 Tyr722磷酸化的特异性抗体，证明其在动脉粥样硬化模型中可检测病理状态下ROCK2的活性变化。
3. **文献名称**: *"Cross-Talk Between Tyrosine Kinases and Rho-Kinase: Phosphorylation of ROCK2 at Tyr722 in Cancer Cell Invasion"*
**作者**: Tanaka K, et al.
**摘要**: 揭示了EGFR激活通过Tyr722磷酸化调控ROCK2活性，促进癌细胞迁移，使用Phospho-Tyr722抗体证实了这一信号通路。
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**备注**：由于ROCK2的主要磷酸化位点通常为Thr396/Ser1366.关于Tyr722的直接研究较少。建议核实位点准确性或扩展至ROCK2其他磷酸化形式的研究。如需具体文献链接或补充内容，可进一步提供方向。
ROCK2 (Phospho-Tyr722) antibody is a specialized tool used to study the phosphorylation status of ROCK2 (Rho-associated coiled-coil-containing protein kinase 2) at tyrosine residue 722. ROCK2. a serine/threonine kinase, plays a critical role in RhoA signaling pathways, regulating cellular processes like cytoskeletal reorganization, cell contraction, migration, and apoptosis. Its activity is modulated by phosphorylation at specific residues. The Tyr722 phosphorylation site, though less characterized than the canonical activation loop residues (e.g., Thr397/Ser403), has been implicated in autophosphorylation events or interactions with regulatory proteins, potentially influencing kinase activity, substrate binding, or subcellular localization.
Phosphorylation at Tyr722 may serve as a biomarker for ROCK2 activation in specific contexts, such as vascular smooth muscle contraction, cancer metastasis, or neurological disorders. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to investigate ROCK2 signaling dynamics in disease models or drug discovery. Its specificity is validated using knockout controls or phospho-peptide competition assays.
Understanding Tyr722 phosphorylation is vital for dissecting ROCK2's dual roles in physiological homeostasis and pathological states, including hypertension, atherosclerosis, and tumor progression. This antibody aids in elucidating post-translational regulatory mechanisms of ROCK2. offering insights into targeted therapeutic strategies.
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