| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2B GL105 antibody; H2B.1 antibody; Histone H2B type 1B antibody; HIST1H2BB antibody |
| Entrez GeneID | 3018; |
| WB Predicted band size | 14kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 32(K-R-S(p)-R-K) derived from Human Histone H2B. |
| Formulation | Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
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以下是关于Histone H2B (Phospho-Ser32) Rabbit抗体的模拟参考文献示例(注：文献为虚构，仅供示例参考)：
1. **文献名称**: *"Phosphorylation of Histone H2B at Serine 32 Regulates DNA Damage-Induced Apoptosis"*
**作者**: Cheung, P. et al.
**摘要**: 本研究揭示了DNA损伤后组蛋白H2B Ser32位点的磷酸化修饰，通过特异性Rabbit抗体检测发现，该修饰通过激活caspase通路促进细胞凋亡，并依赖ATM激酶的活性。
2. **文献名称**: *"A Novel Role of H2B Ser32 Phosphorylation in Chromatin Remodeling During Mitosis"*
**作者**: Ahn, J. & Lee, S.
**摘要**: 作者利用Phospho-Ser32 H2B抗体进行免疫荧光和ChIP实验，证明该修饰在有丝分裂早期调控染色体凝聚，并与Aurora B激酶活性相关。
3. **文献名称**: *"AMPK-Mediated Phosphorylation of Histone H2B at Ser32 Links Metabolic Stress to Gene Repression"*
**作者**: Martinez, R. et al.
**摘要**: 研究发现能量应激(如葡萄糖剥夺)通过AMPK激活H2B Ser32磷酸化，利用该抗体验证其与特定基因启动子的结合，导致转录抑制。
4. **文献名称**: *"Validation of a Phospho-Specific Antibody for Histone H2B (Ser32) in Epigenetic Studies"*
**作者**: Dogan, T. et al.
**摘要**: 文章详细验证了该抗体的特异性(通过肽段竞争实验)及其在Western blot和免疫组化中的应用，为后续表观遗传机制研究提供工具支持。
**注意**：以上文献为模拟生成，实际引用时请通过PubMed或Google Scholar检索真实研究。建议使用关键词 **"H2B Ser32 phosphorylation"** 或 **"Histone H2B (Phospho-Ser32) antibody application"** 进一步查找。
The Histone H2B (Phospho-Ser32) Rabbit antibody is a specialized tool designed to detect the phosphorylation of histone H2B at serine residue 32 (Ser32), a post-translational modification implicated in chromatin dynamics and cellular processes. Histone H2B, a core component of nucleosomes, plays a critical role in DNA packaging and gene regulation. Phosphorylation of histones, including H2B, is a key regulatory mechanism influencing DNA repair, transcriptional activation, and chromatin remodeling during mitosis or apoptosis.
Phosphorylation at Ser32 in H2B has been associated with cellular responses to DNA damage and mitotic progression. This modification may act as a signaling marker, recruiting repair proteins or facilitating chromatin structural changes. The antibody specifically recognizes the phosphorylated form of H2B at Ser32. enabling researchers to study its spatial and temporal distribution under various physiological or stress conditions.
Validated for applications like Western blotting, immunofluorescence, and immunohistochemistry, this rabbit-derived polyclonal antibody offers high specificity and sensitivity. It is commonly used in epigenetics and cancer research to explore mechanisms of genomic instability, cell cycle regulation, and apoptosis. Proper storage conditions (e.g., -20°C) and optimized experimental protocols are recommended to ensure reliable detection of this transient and low-abundance modification.
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