| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FCC2; COCA2; HNPCC |
| Entrez GeneID | 4292 |
| clone | 4C9C7 |
| WB Predicted band size | 85kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Histone H3(Phospho-Ser28)抗体的3篇示例参考文献(内容为示例，非真实文献)：
1. **《Mitotic Phosphorylation of Histone H3 at Serine 28 Mediates Chromosome Condensation》**
- 作者：Hirota, T. et al.
- 摘要：研究揭示了Aurora B激酶通过磷酸化Histone H3的Ser28位点，驱动有丝分裂期染色体凝聚的分子机制，该抗体用于检测磷酸化修饰水平。
2. **《DNA Damage-Induced Phosphorylation of Histone H3(Ser28) in Human Cells》**
- 作者：Cheung, P. & Allis, C.D.
- 摘要：探讨DNA损伤应激下，ATM/ATR激酶介导的H3 Ser28磷酸化在染色质重塑中的作用，抗体用于免疫荧光定位分析。
3. **《H3S28 Phosphorylation as a Biomarker for Cancer Progression》**
- 作者：Li, J. et al.
- 摘要：通过免疫组化分析多种肿瘤样本，发现Histone H3(Phospho-Ser28)的高表达与胶质瘤侵袭性相关，提示其作为预后标志物的潜力。
4. **《Epigenetic Regulation by H3S28 Phosphorylation in Embryonic Stem Cells》**
- 作者：Bibikova, M. et al.
- 摘要：研究H3 Ser28磷酸化在小鼠胚胎干细胞分化中的动态变化，抗体用于ChIP-seq实验揭示其与基因沉默区域的关联。
(注：以上文献为模拟示例，实际引用需查阅真实数据库如PubMed。)
The Histone H3 (Phospho-Ser28) Rabbit antibody is a specific immunological tool designed to detect histone H3 protein phosphorylated at serine 28 (Ser28), a post-translational modification (PTM) associated with chromatin dynamics and transcriptional regulation. Histone H3 is a core component of nucleosomes, playing a critical role in DNA packaging and epigenetic regulation. Phosphorylation at Ser28. located in the N-terminal tail of histone H3. is linked to mitotic chromosome condensation and segregation during cell division. This modification is dynamically regulated by kinases such as Aurora B and other mitotic kinases, and its levels peak during metaphase.
The antibody is commonly used in techniques like Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC) to study cell cycle progression, chromatin remodeling, and DNA damage responses. Its specificity for the phosphorylated Ser28 epitope enables researchers to distinguish this modification from other histone H3 phosphorylation sites (e.g., Ser10 or Thr3). Validation typically includes knockout controls or peptide competition assays to confirm target specificity.
Applications span cancer research, developmental biology, and epigenetics, as aberrant histone H3 phosphorylation is implicated in genomic instability and oncogenesis. The rabbit-derived antibody offers high affinity and reproducibility, making it a key reagent for exploring mitotic regulation and chromatin-based mechanisms in health and disease.
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