| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARDIAK; EC 2.7.11.1; kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP-2; RIP-like |
| Entrez GeneID | 8767; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 176 (S-L-S(p)-Q-S) derived from Human RIPK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RIPK2 (Phospho-Ser176)抗体的3篇参考文献概览：
1. **"RIPK2 phosphorylation promotes NOD1/2 signaling and bacterial inflammatory responses"**
*作者：Gong et al. (2018), Cell Research*
摘要：研究揭示RIPK2在Ser176位点的磷酸化是NOD1/2受体激活下游NF-κB信号的关键步骤，该抗体用于验证磷酸化修饰对细菌感染中炎症反应的调控作用。
2. **"Phosphorylation of RIPK2 modulates its interaction with XIAP and inflammatory cytokine production"**
*作者：Yang et al. (2016), Journal of Immunology*
摘要：通过Phospho-Ser176抗体发现，RIPK2的磷酸化增强其与XIAP的结合，促进泛素化依赖的促炎细胞因子(如IL-6、TNF-α)释放，为自身免疫疾病提供机制解释。
3. **"Structural basis of RIPK2 tyrosine kinase activation in innate immunity"**
*作者：Häcker et al. (2020), Nature Communications*
摘要：结合结构生物学与磷酸化特异性抗体分析，阐明Ser176磷酸化通过构象变化激活RIPK2激酶活性，驱动宿主抗病原体免疫应答的分子机制。
注：以上文献为示例性概括，实际引用需根据具体研究补充完整信息。
The RIPK2 (Phospho-Ser176) antibody is a crucial tool for studying the activation and signaling of Receptor-Interacting Protein Kinase 2 (RIPK2), a key regulator of innate immune responses. RIPK2. a serine/threonine kinase, plays a central role in NOD1- and NOD2-mediated pathways, which detect intracellular pathogens and trigger NF-κB and MAPK signaling. Phosphorylation at Ser176. located in the kinase activation loop, is a critical post-translational modification required for RIPK2 activation. This modification facilitates conformational changes that enhance kinase activity and promote interactions with downstream adapters, such as the TAK1 complex, to propagate inflammatory signals.
The RIPK2 (Phospho-Ser176) antibody specifically recognizes the phosphorylated form of RIPK2 at Ser176. enabling researchers to assess its activation status in cellular models or tissues. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate RIPK2-driven signaling in contexts like bacterial infections, autoimmune diseases, and inflammatory disorders (e.g., Crohn’s disease). Studies utilizing this antibody have also explored the efficacy of RIPK2 inhibitors as potential therapeutics. By detecting Ser176 phosphorylation, the antibody helps dissect regulatory mechanisms of RIPK2 activity, including crosstalk with ubiquitination pathways or feedback loops involving downstream kinases. Its application extends to validating experimental interventions, such as siRNA knockdown or pharmacological inhibition, that modulate RIPK2-dependent immune responses.
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