| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA0827; NF-AT5; nuclear factor of activated T cells 5; T cell transcription factor NFAT5; TonE-binding protein; TonEBP; tonicity-responsive enhancer-binding protein |
| Entrez GeneID | 10725; |
| WB Predicted band size | 200kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of serine 155 (D-N-S(p)-R-M) derived from Human NFAT5/TonEBP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下为3篇关于NFAT5/TonEBP (Phospho-Ser155)抗体的参考文献示例(注：部分内容为模拟概括，建议通过PubMed等数据库核实具体文献)：
---
1. **"TonEBP/NFAT5 phosphorylation regulates hyperosmotic stress-induced transcriptional activation"**
- **作者**: Lee, J.H. et al.
- **摘要**: 研究揭示了Ser155磷酸化对TonEBP在高渗应激下核转位及转录活性的关键作用，使用Phospho-Ser155特异性抗体通过Western blot和免疫荧光验证了该位点的修饰与细胞渗透压适应的关系。
2. **"Phosphorylation of NFAT5 at Ser155 modulates renal medullary solute transport"**
- **作者**: Roth, I. et al.
- **摘要**: 通过Phospho-Ser155抗体检测肾脏髓质细胞中NFAT5的磷酸化水平，证明该修饰通过调控尿素转运蛋白表达参与维持肾髓质高渗环境，为慢性肾脏病机制提供新见解。
3. **"Hypertonicity-induced phosphorylation of TonEBP/NFAT5 requires p38 MAPK signaling"**
- **作者**: Zhou, X. et al.
- **摘要**: 利用Phospho-Ser155抗体证实p38 MAPK通路介导的高渗应激下TonEBP磷酸化，并揭示其在炎症因子(如TNF-α)表达调控中的下游效应，为炎症相关疾病治疗提供靶点。
---
**注**：以上文献标题和内容为示例性质，实际研究中建议通过关键词“NFAT5 Phospho-Ser155 antibody”在学术数据库检索最新文献。
**Background of NFAT5/TonEBP (Phospho-Ser155) Antibody**
NFAT5 (Nuclear Factor of Activated T-cells 5), also known as TonEBP (Tonicity-Responsive Enhancer-Binding Protein), is a transcription factor critical for cellular adaptation to hyperosmotic stress. It regulates genes involved in osmoprotective responses, such as sodium/myo-inositol transporters (SMIT), aldose reductase (AR), and betaine/GABA transporters (BGT1), which help restore cell volume and homeostasis under high osmotic conditions. Beyond osmoregulation, NFAT5/TonEBP is implicated in immune responses, inflammation, and cancer progression.
Phosphorylation at Serine 155 (Ser155) is a key post-translational modification modulating NFAT5 activity. This phosphorylation event, mediated by kinases like p38 MAPK or ATM/ATR, enhances NFAT5 nuclear translocation, DNA-binding affinity, and transcriptional activation. It serves as a marker of NFAT5 activation under osmotic stress or DNA damage.
The NFAT5/TonEBP (Phospho-Ser155) antibody specifically detects this phosphorylated form, enabling researchers to study NFAT5 activation dynamics in various contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate cellular stress responses, inflammatory pathways, and tumor microenvironments. Dysregulation of NFAT5 has been linked to diseases such as diabetes, neurodegeneration, and cancer, making this antibody a valuable tool for exploring therapeutic targets. Validation of this antibody typically includes testing in knockout models or phosphorylation-blocking assays to ensure specificity.
×
