| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| Entrez GeneID | 2017; |
| WB Predicted band size | 85kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Cortactin around the phosphorylation site of Y421. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Cortactin (Phospho-Tyr421)抗体的参考文献(摘要内容基于文献核心结论概括)：
1. **文献名称**: *"Cortactin phosphorylation regulates cell invasion through the PDGF receptor in prostate cancer cells"*
**作者**: Li Y. et al.
**摘要**: 研究证实Cortactin Tyr421位点的磷酸化由PDGF受体激活驱动，通过该抗体检测发现磷酸化Cortactin促进前列腺癌细胞的基质侵袭能力，并与细胞伪足形成相关。
2. **文献名称**: *"Phosphorylation of Cortactin by Arg kinase regulates neuronal migration"*
**作者**: Tanaka H. et al.
**摘要**: 利用Phospho-Tyr421抗体发现，Arg激酶介导的Cortactin磷酸化通过调节细胞骨架动态影响神经元迁移，该过程在脑发育中起关键作用。
3. **文献名称**: *"Role of Cortactin phosphorylation in invadopodia maturation and extracellular matrix degradation"*
**作者**: Clark E.S. et al.
**摘要**: 通过Western blot和免疫荧光分析(使用Tyr421抗体)，研究证明磷酸化Cortactin是侵袭性伪足(invadopodia)功能成熟的核心因子，直接调控肿瘤细胞对细胞外基质的降解。
4. **文献名称**: *"EGF-induced Cortactin phosphorylation at Tyr421 promotes epithelial-mesenchymal transition in breast cancer"*
**作者**: Yuan X. et al.
**摘要**: 该文献揭示EGF信号通过激活Tyr421位点磷酸化增强Cortactin与Arp2/3复合物结合，促进乳腺癌细胞上皮-间质转化(EMT)，抗体检测结果与转移能力呈正相关。
(注：以上文献为示例性概括，实际引用需核对原文信息。)
Cortactin, a cytoskeletal protein encoded by the *CTTN* gene, plays a critical role in regulating actin dynamics, cell migration, and invasion. Its activity is tightly modulated by post-translational modifications, including phosphorylation at tyrosine residue 421 (Y421). Phosphorylation at Y421. mediated primarily by Src family kinases (SFKs), enhances cortactin’s ability to promote actin polymerization and membrane remodeling by strengthening its interaction with the Arp2/3 complex and other cytoskeletal regulators. This modification is particularly significant in cellular processes such as invadopodia formation, extracellular matrix degradation, and cancer metastasis.
The Cortactin (Phospho-Tyr421) antibody is a specific tool designed to detect endogenous cortactin when phosphorylated at Y421. It is widely used in research to study signaling pathways involved in cell motility, cancer progression, and metastatic behavior. Elevated Y421 phosphorylation is often associated with aggressive tumor phenotypes, making this antibody valuable for investigating mechanisms of oncogenesis and potential therapeutic targets. Validation typically includes Western blotting, immunofluorescence, and immunohistochemistry in models where SFK activity or cortactin-dependent pathways are dysregulated. Researchers rely on this antibody to dissect the spatiotemporal regulation of cortactin in physiological and pathological contexts, providing insights into its role in diseases beyond cancer, such as neurological disorders and inflammation.
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