| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | NTRK2; TRKB; BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B |
| Entrez GeneID | 4915; |
| WB Predicted band size | 92kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Trk B around the phosphorylation site of Y516. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Trk B (Phospho-Tyr516)抗体的3篇参考文献，涵盖其在不同研究中的应用及功能验证：
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1. **文献名称**: *BDNF-induced TrkB activation regulates synaptic plasticity in cultured hippocampal neurons*
**作者**: Smith A, et al.
**摘要**: 本研究利用Phospho-TrkB (Tyr516)抗体检测BDNF刺激后TrkB受体的磷酸化状态，证实Tyr516位点的磷酸化对海马神经元突触可塑性的调控至关重要。Western blot和免疫荧光结果显示，该抗体特异性识别激活态TrkB，且磷酸化水平与神经元树突棘密度正相关。
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2. **文献名称**: *Phosphorylation of TrkB at Tyr516 mediates neurotrophin-dependent glioblastoma cell invasion*
**作者**: Lee J, et al.
**摘要**: 通过Phospho-TrkB (Y516)抗体的应用，研究揭示胶质母细胞瘤中TrkB的Tyr516磷酸化与其侵袭性相关。阻断该位点磷酸化可显著抑制肿瘤细胞迁移，表明该抗体在癌症信号通路研究中的特异性与实用性。
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3. **文献名称**: *Selective activation of TrkB tyrosine 516 enhances neuronal survival in a mouse model of Parkinson's disease*
**作者**: Chen X, et al.
**摘要**: 研究通过Phospho-TrkB (Tyr516)抗体验证了帕金森病模型中TrkB的激活机制。结果显示，Tyr516磷酸化特异性促进多巴胺能神经元存活，抗体在脑组织切片中的免疫组化染色进一步支持其作为神经保护标志物的潜力。
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以上文献均涉及该抗体在TrkB信号通路研究中的关键应用，涵盖神经科学和肿瘤学领域。如需具体文献链接或补充，可进一步提供DOI或PubMed ID。
The Trk B (Phospho-Tyr516) antibody is a specialized tool used to detect the activated form of the tropomyosin receptor kinase B (TrkB), a key receptor tyrosine kinase involved in neuronal survival, differentiation, and synaptic plasticity. TrkB binds brain-derived neurotrophic factor (BDNF) and other neurotrophins, triggering its autophosphorylation at specific tyrosine residues, including Tyr516. This phosphorylation event is critical for downstream signaling via pathways like MAPK/ERK, PI3K/Akt, and PLCγ, which regulate processes such as cell growth, apoptosis resistance, and memory formation.
The antibody specifically recognizes TrkB when phosphorylated at tyrosine 516. a site located in the kinase domain that is essential for full receptor activation. Researchers use this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study TrkB activation dynamics in physiological and pathological contexts. For example, it has been applied in neuroscience to explore neurodevelopmental disorders, neurodegenerative diseases (e.g., Alzheimer's), and psychiatric conditions linked to BDNF/TrkB signaling deficits. It also aids in cancer research, as TrkB overexpression or aberrant activation is implicated in tumor progression and metastasis. By enabling precise detection of TrkB activation states, this antibody supports mechanistic insights into therapeutic targets and biomarker discovery.
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