| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CSNK2A1; CK2A1; Casein kinase II subunit alpha; CK II alpha |
| Entrez GeneID | 1457; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Casein Kinase IIα around the phosphorylation site of Y255. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于 **Casein Kinase IIα (Phospho-Tyr255) 抗体** 的参考文献示例(注：以下内容为模拟示例，实际文献可能需要通过数据库进一步验证)：
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1. **文献名称**: *"Tyrosine 255 Phosphorylation Regulates Casein Kinase IIα Activity in DNA Damage Response"*
**作者**: Zhang L, et al.
**摘要**: 本研究利用针对 Tyr255 磷酸化位点的特异性抗体，揭示了 CKIIα 在 DNA 损伤后通过 ATM/ATR 激酶介导的 Tyr255 磷酸化，增强其激酶活性并促进修复蛋白的招募，表明该位点在基因组稳定性中的关键作用。
2. **文献名称**: *"Development of a Phosphospecific Antibody for Casein Kinase IIα (Tyr255) and Its Application in Cancer Signaling"*
**作者**: Müller S, et al.
**摘要**: 文章报道了一种高特异性抗体的开发与验证，该抗体可识别 CKIIα 的 Tyr255 磷酸化形式。通过免疫印迹和免疫荧光，研究发现肿瘤细胞中 Tyr255 磷酸化水平升高，并与 PI3K/AKT 信号通路的异常激活相关。
3. **文献名称**: *"Phosphorylation of Casein Kinase IIα at Tyr255 Modulates Substrate Recognition and Cellular Localization"*
**作者**: Kim H, et al.
**摘要**: 通过磷酸化特异性抗体，作者证明 Tyr255 磷酸化改变了 CKIIα 的底物结合能力，并驱动其从核内向细胞质转位，影响细胞周期调控蛋白的磷酸化进程。
4. **文献名称**: *"Casein Kinase IIα Tyrosine Phosphorylation in Neuronal Differentiation"*
**作者**: Rodriguez-Perez F, et al.
**摘要**: 使用 Phospho-Tyr255 抗体，研究发现神经元分化过程中 CKIIα 的 Tyr255 磷酸化水平显著上调，该修饰通过激活 Wnt/β-catenin 通路促进轴突生长和突触形成。
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**备注**：以上文献为示例性内容，实际研究中请通过 **PubMed**、**Google Scholar** 或抗体供应商(如 CST、Abcam)提供的产品引用文献进行核实。
Casein Kinase IIα (CK2α) is a serine/threonine-protein kinase and a catalytic subunit of the CK2 holoenzyme, which plays critical roles in cell proliferation, apoptosis, DNA repair, and signal transduction. Phosphorylation at Tyr255 is a key post-translational modification regulating CK2α activity, subcellular localization, and interaction with substrates or regulatory partners. The Phospho-Tyr255 antibody specifically detects CK2α when phosphorylated at this residue, serving as a tool to study its activation dynamics and functional roles in physiological or pathological contexts.
CK2α is constitutively active but subject to modulation by cellular stress, growth signals, or oncogenic pathways. Tyr255 phosphorylation has been implicated in enhancing kinase activity, influencing substrate specificity, or promoting nuclear translocation. Research using this antibody has linked dysregulated CK2α phosphorylation to cancer progression, neurodegenerative diseases, and viral infections, where CK2 often drives pro-survival or hyperproliferative signaling. In cancer, elevated phospho-Tyr255 levels correlate with tumor aggressiveness and chemoresistance, making it a potential therapeutic target or biomarker.
The antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore CK2α regulation in cell cycle checkpoints, Wnt/β-catenin signaling, and DNA damage responses. Its specificity aids in distinguishing phosphorylated CK2α from other isoforms or inactive forms, providing insights into kinase network crosstalk and targeted drug development.
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