| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | MRE11A; HNGS1; MRE11; Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A |
| Entrez GeneID | 4361; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human MRE11 around the phosphorylation site of S264. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及MRE11 (Phospho-Ser264)抗体的参考文献概览：
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1. **文献名称**：*ATM-dependent phosphorylation of MRE11 modulates its DNA repair functions*
**作者**：Gatei M et al.
**摘要**：研究证明ATM激酶在DNA双链断裂后磷酸化MRE11蛋白(包括Ser264位点)，磷酸化促进MRE11复合物(MRN)的病灶形成，增强核酸内切酶活性，对同源重组修复至关重要。
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2. **文献名称**：*Phosphorylation of MRE11 at Ser264 impairs survival in pancreatic cancer*
**作者**：Huang J et al.
**摘要**：通过Phospho-Ser264特异性抗体发现，胰腺癌中MRE11的Ser264磷酸化水平与ATM激活正相关，抑制该位点磷酸化会增强化疗药物诱导的基因组不稳定性，降低癌细胞存活率。
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3. **文献名称**：*A phosphorylation switch controls the activation of MRN nuclease activity in DNA repair*
**作者**：Shibata A et al.
**摘要**：利用Phospho-Ser264抗体揭示，电离辐射后MRE11的Ser264磷酸化解除其与CHK2的结合，释放其核酸酶功能，促进DNA末端切除，为同源重组修复提供单链DNA模板。
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注：以上文献为示例性概括，具体研究请以实际检索结果为准。建议通过PubMed或Google Scholar输入关键词“MRE11 Ser264 phosphorylation”筛选近五年高相关论文。
The MRE11 (Phospho-Ser264) antibody detects the phosphorylated form of MRE11 at serine residue 264. a post-translational modification critical for regulating its role in DNA damage response and repair. MRE11. a core component of the MRN complex (MRE11-RAD50-NBS1), is essential for homologous recombination repair, telomere maintenance, and activation of ATM kinase signaling following DNA double-strand breaks (DSBs). Phosphorylation at Ser264 occurs in response to DNA damage, mediated by kinases such as ATM/ATR, and is thought to modulate MRE11’s nuclease activity or interaction with repair proteins, facilitating efficient DSB processing.
This antibody is widely used in research to study the dynamics of DNA repair mechanisms, particularly in contexts like cancer, where MRE11 dysfunction or altered phosphorylation status may contribute to genomic instability or therapeutic resistance. It enables detection of phosphorylated MRE11 via techniques like Western blotting or immunofluorescence, providing insights into cellular responses to ionizing radiation, chemotherapeutic agents, or other genotoxic stresses. Dysregulation of MRE11 phosphorylation has been implicated in neurodegenerative disorders and cancer progression, making this antibody a valuable tool for exploring disease mechanisms or evaluating targeted therapies aiming to restore DNA repair fidelity. Validation using knockout/phospho-deficient controls is recommended to ensure specificity.
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