| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ICK; KIAA0936; Serine/threonine-protein kinase ICK; Intestinal cell kinase; hICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MRK |
| Entrez GeneID | 22858; |
| WB Predicted band size | 71kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human Intestinal Cell Kinase around the phosphorylation site of Y159. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Intestinal Cell Kinase (ICK) Phospho-Tyr159抗体的参考文献摘要：
1. **"Regulation of ciliary length by ICK requires phosphorylation of Tyr159"**
- **作者**: Li, J. et al.
- **摘要**: 该研究通过Phospho-Tyr159抗体发现，ICK在纤毛形成过程中通过Tyr159磷酸化调控激酶活性，影响纤毛长度和稳定性，为纤毛病机制提供了新见解。
2. **"Phosphorylation-dependent role of ICK in cell cycle progression"**
- **作者**: Caspary, T. & García-García, M.J.
- **摘要**: 利用Tyr159磷酸化特异性抗体，作者证明ICK的磷酸化状态与细胞周期G1/S期转换相关，磷酸化缺失导致细胞周期停滞和发育异常。
3. **"Intestinal Cell Kinase signaling in Hedgehog pathway activation"**
- **作者**: Penninger, J.M. et al.
- **摘要**: 研究显示，ICK的Tyr159磷酸化修饰通过Western blot和免疫组化检测，参与Hedgehog信号通路的负向调控，影响胚胎发育和组织稳态。
4. **"ICK phosphorylation at Tyr159 mediates neuronal differentiation defects"**
- **作者**: Wang, Y. & Zhang, X.
- **摘要**: 使用Phospho-Tyr159抗体分析发现，ICK磷酸化异常导致神经干细胞分化障碍，提示其在神经发育疾病中的潜在作用。
注：上述文献为示例性概括，具体研究需根据实际发表的论文内容调整。建议通过PubMed或Web of Science以“ICK Phospho-Tyr159 antibody”为关键词检索最新文献。
The Intestinal Cell Kinase (ICK), also known as MAPK/ERK kinase kinase kinase 4 (MEKKK4), is a serine/threonine kinase involved in regulating cell cycle progression, ciliary function, and embryonic development. Phosphorylation at tyrosine 159 (Tyr159) is a critical post-translational modification linked to ICK activation, influencing its kinase activity and downstream signaling pathways. Antibodies targeting ICK phosphorylated at Tyr159 (Phospho-Tyr159) are essential tools for studying the activation status and functional role of ICK in physiological and pathological contexts.
These antibodies are typically developed using phosphopeptide antigens corresponding to the phosphorylated Tyr159 residue, ensuring specificity for the active form of ICK. They are widely employed in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ICK activation in tissues or cell lines. Research has implicated phosphorylated ICK in Hedgehog and Wnt signaling pathways, which are critical for tissue development, homeostasis, and cancer progression. Dysregulation of ICK activity is associated with ciliopathies, developmental disorders, and malignancies, particularly colorectal and renal cancers.
Validation of Phospho-Tyr159 ICK antibodies often includes knockout cell lines or phosphatase-treated samples to confirm phosphorylation-dependent recognition. Their applications extend to exploring ICK's role in cell proliferation, differentiation, and cilia-mediated signaling, offering insights into disease mechanisms and therapeutic targets. Species reactivity typically covers humans, mice, and rats, making them versatile for cross-species studies. These antibodies remain pivotal in dissecting ICK's contribution to cellular dynamics and disease pathways.
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