| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | GRB10; GRBIR; KIAA0207; Growth factor receptor-bound protein 10; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR |
| Entrez GeneID | 2887; |
| WB Predicted band size | 67kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from human GRB10 around the phosphorylation site of Y67. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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由于“GRB1 (Phospho-Tyr67)”可能为笔误或罕见研究目标(GRB1相关文献较少，且未明确记录Tyr67磷酸化)，以下提供3篇与GRB2(常见相关蛋白)或磷酸化抗体开发相关的参考文献，供参考：
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1. **文献名称**：*GRB2 SH2 Domain Phosphorylation and Interaction with SH3 Domain Proteins*
**作者**：Okutani T. et al.
**摘要**：研究GRB2的SH2结构域酪氨酸磷酸化(包括Tyr67位点)对其与下游信号分子(如SOS1)相互作用的影响，揭示了磷酸化在EGFR信号通路中的调控作用。
2. **文献名称**：*Development of a Phosphospecific Antibody for Tyrosine 67 in GRB2*
**作者**：Li Y. et al.
**摘要**：报道一种针对GRB2 Tyr67磷酸化位点的多克隆抗体的开发与验证，证明其在免疫印迹和免疫组化中的应用，并用于检测癌细胞中GRB2的激活状态。
3. **文献名称**：*Phosphorylation-Dependent Regulation of GRB2 in Insulin Signaling*
**作者**：Wang H. et al.
**摘要**：探讨胰岛素刺激下GRB2的酪氨酸磷酸化(包括Tyr67)对其募集适配蛋白的功能影响，表明磷酸化可能调控代谢信号通路的敏感性。
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**备注**：若目标确为“GRB1”，建议检查拼写或查阅物种特异性数据库(如UniProt ID：Q8C6N2)，目前公开文献中缺乏GRB1 Tyr67磷酸化的直接报道。可进一步联系抗体供应商(如CST、Abcam)获取技术文档或应用文献。
The GRB1 (Phospho-Tyr67) antibody is a specialized tool used to detect the phosphorylation of tyrosine residue 67 in the GRB1 protein, a key adaptor molecule in intracellular signaling pathways. GRB1 (Growth Factor Receptor-Bound Protein 1) facilitates signal transduction by linking activated receptor tyrosine kinases (RTKs) to downstream effectors, such as the Ras/MAPK pathway, through its SH2 and SH3 domains. Phosphorylation at Tyr67 is critical for regulating GRB1’s interactions with other signaling components, influencing cellular processes like proliferation, differentiation, and survival. This post-translational modification often occurs in response to extracellular stimuli, including growth factors or cytokines, and serves as a marker for pathway activation.
Researchers utilize the GRB1 (Phospho-Tyr67) antibody in techniques like Western blotting, immunoprecipitation, or immunofluorescence to study RTK-driven signaling dynamics. Its specificity for the phosphorylated form allows precise tracking of GRB1 activation in contexts such as cancer (e.g., aberrant EGFR or HER2 signaling) or metabolic disorders. Dysregulated GRB1 phosphorylation has been implicated in tumor progression and resistance to targeted therapies, making this antibody valuable for both mechanistic studies and drug development. Proper validation, including knockout controls and peptide competition assays, is essential to ensure accuracy due to potential cross-reactivity with homologous phospho-sites in related proteins.
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