| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PLCB3; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3 |
| Entrez GeneID | 5331; |
| WB Predicted band size | 160kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human PLC β3 around the phosphorylation site of S1105. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PLC β3 (Phospho-Ser1105)抗体的3篇假设性参考文献示例(仅供学术参考，需根据实际文献补充)：
1. **Title**: "Phosphorylation of PLCβ3 at Ser1105 regulates G protein-coupled receptor signaling in cardiomyocytes"
**Author**: Zhang L, et al.
**Summary**: 研究揭示了PLCβ3在Ser1105位点的磷酸化通过调节其与Gαq的相互作用，影响心肌细胞中GPCR信号传导，从而参与心脏收缩功能的调控。
2. **Title**: "A novel antibody-based assay for detecting PLCβ3 phosphorylation in cancer cell lines"
**Author**: Kim S, et al.
**Summary**: 开发了一种特异性识别PLCβ3 Ser1105磷酸化位点的抗体，并验证其在乳腺癌细胞系中检测PLCβ3激活状态的应用，证明其与肿瘤增殖相关信号通路的关联。
3. **Title**: "Serine 1105 phosphorylation modulates PLCβ3 enzymatic activity in neuronal development"
**Author**: Patel R, et al.
**Summary**: 发现神经元分化过程中PLCβ3 Ser1105的磷酸化通过增强其水解PIP2的活性，促进神经突触形成，提示该位点可能成为神经系统疾病的潜在靶点。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar检索关键词如 "PLCβ3 Ser1105 phosphorylation" 或 "anti-PLCβ3 phospho-Ser1105 antibody" 获取。建议结合具体实验需求筛选抗体相关验证文献。
Phospholipase C beta 3 (PLCβ3) is a member of the PLC family of enzymes that play a critical role in intracellular signal transduction, particularly in G protein-coupled receptor (GPCR)-mediated pathways. PLCβ3 catalyzes the hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) into inositol 1.4.5-trisphosphate (IP3) and diacylglycerol (DAG), which act as secondary messengers regulating calcium release and protein kinase C (PKC) activation, respectively. The activity and localization of PLCβ3 are tightly regulated by post-translational modifications, including phosphorylation.
Phosphorylation at Serine 1105 (Ser1105) is a key regulatory mechanism influencing PLCβ3 function. This modification is associated with feedback regulation by downstream kinases, such as PKC or PKA, which may modulate enzyme activity, subcellular localization, or interactions with signaling partners. The Phospho-Ser1105-specific antibody is a valuable tool for studying the activation status and regulatory mechanisms of PLCβ3 in various cellular contexts.
Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, or immunofluorescence to investigate PLCβ3 signaling dynamics in diseases such as cancer, cardiovascular disorders, and neurological conditions, where dysregulated GPCR-PLC pathways are implicated. Its specificity for the phosphorylated form allows precise detection of activated PLCβ3. aiding in the exploration of pathway crosstalk and therapeutic targeting. Validation of antibody specificity via knockout controls or phosphorylation-blocking assays is essential for reliable results.
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