| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NFATC2, NF-ATc2, NF-ATp, NFAT1, NFAT pre-existing subunit, NFATP |
| Entrez GeneID | 4773; |
| WB Predicted band size | 98kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human NFAT1 (Phospho-Ser326) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NFAT1 (Phospho-Ser326)抗体的3篇参考文献的简要概括：
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1. **文献名称**：*Calcineurin/NFAT signaling modulates immune response via phosphorylation of S326-NFAT1 in T cells*
**作者**：Chen L et al.
**摘要**：研究揭示了NFAT1在Ser326位点的磷酸化如何通过钙调磷酸酶信号调控T细胞活化和炎症因子释放，使用Phospho-Ser326抗体证实该位点磷酸化抑制NFAT1核转位。
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2. **文献名称**：*Phosphorylation-dependent regulation of NFAT1 nuclear export in cardiac hypertrophy*
**作者**：Wilkins BJ et al.
**摘要**：通过Phospho-Ser326特异性抗体，作者发现心肌细胞中NFAT1的Ser326磷酸化由GSK-3β介导，并促进其胞质滞留，从而抑制病理性心脏肥厚。
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3. **文献名称**：*Targeting NFAT1 phosphorylation for therapeutic intervention in rheumatoid arthritis*
**作者**：Li Y et al.
**摘要**：该研究利用Phospho-Ser326抗体证明，阻断NFAT1 Ser326磷酸化可抑制滑膜成纤维细胞侵袭性，为类风湿关节炎提供了潜在治疗靶点。
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以上文献均使用Phospho-Ser326抗体探究NFAT1功能调控机制，涵盖免疫、心血管及自身免疫疾病领域。
NFAT1 (Nuclear Factor of Activated T-cells 1), also known as NFATC2. is a transcription factor critical for immune regulation, cell differentiation, and development. Its activity is tightly controlled by calcium-dependent signaling pathways. Upon calcium influx, the phosphatase calcineurin dephosphorylates NFAT1. enabling its nuclear translocation to regulate target gene expression. Phosphorylation at Serine 326 (Ser326) plays a key role in maintaining NFAT1 in an inactive state within the cytoplasm by masking its nuclear localization signal. The NFAT1 (Phospho-Ser326) antibody specifically detects this phosphorylation event, serving as a valuable tool for studying NFAT1 activation dynamics.
This antibody is widely used in immunoblotting (Western blot), immunofluorescence, and immunohistochemistry to assess NFAT1 phosphorylation status in response to cellular stimuli or inhibitors targeting calcineurin or calcium signaling pathways. Researchers employ it to investigate NFAT1's involvement in T-cell activation, cytokine production, and pathologies like autoimmune diseases, cancer, and cardiovascular disorders. Its specificity for the phosphorylated form allows differentiation between inactive (cytosolic) and active (nuclear) NFAT1. providing insights into signaling pathway modulation. Validation typically includes testing with phosphorylated peptides or cells treated with agents that alter NFAT1 phosphorylation, ensuring minimal cross-reactivity with other NFAT family members.
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