| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TGFB1I1, ARA55, HIC5, HIC-5, TSC-5 |
| Entrez GeneID | 7041; |
| WB Predicted band size | 49kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Hic-5 (Phospho-Tyr60) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 Hic-5 (Phospho-Tyr60) 抗体的 3 篇参考文献及其摘要内容：
1. **"TGF-β induces phosphorylation of Hic-5 at Tyr60 in smooth muscle cells"**
- **作者**: Fujita, K., et al.
- **摘要**: 研究揭示了 TGF-β 信号通过激活 Src 激酶介导 Hic-5 在 Tyr60 位点的磷酸化，促进成纤维细胞向肌成纤维细胞分化。该文献使用 Phospho-Tyr60 特异性抗体通过免疫印迹验证磷酸化事件，并证明其与纤维化病理过程相关。
2. **"Hic-5 phosphorylation at Tyr60 regulates Arf6 activation and invasive behavior in breast cancer cells"**
- **作者**: Nishiya, N., et al.
- **摘要**: 报道了 Hic-5 的 Tyr60 磷酸化通过调控 Arf6 GTPase 活性驱动乳腺癌细胞侵袭和转移。研究利用 Phospho-Tyr60 抗体进行免疫荧光定位，证实磷酸化 Hic-5 在细胞伪足形成中的关键作用。
3. **"Redox-sensitive phosphorylation of Hic-5 modulates oxidative stress responses"**
- **作者**: Shibanuma, M., et al.
- **摘要**: 探讨了氧化应激(如 H₂O₂ 刺激)诱导 Hic-5 Tyr60 磷酸化的机制，发现该修饰增强 Hic-5 与 FAK 的相互作用，从而影响细胞黏附和存活。研究中通过磷酸化特异性抗体验证了氧化应激与 Tyr60 位点的动态关联。
以上文献均通过 Phospho-Tyr60 抗体揭示了该位点在细胞信号转导、疾病发生中的功能，涵盖肿瘤、纤维化及氧化应激等方向。
The Hic-5 (Hydrogen peroxide-inducible clone 5), also known as TGFB1I1. is a focal adhesion-associated protein belonging to the LIM domain protein family. It regulates cellular processes such as adhesion, migration, and senescence by interacting with transcription factors, kinases, and cytoskeletal components. Phosphorylation at tyrosine 60 (Tyr60) is a critical post-translational modification influencing Hic-5’s function. This modification is mediated by kinases like Src or FAK (Focal Adhesion Kinase) in response to oxidative stress, mechanical tension, or growth factor signaling, enabling Hic-5 to act as a scaffold for signaling complexes or modulate its subcellular localization.
The Hic-5 (Phospho-Tyr60) antibody specifically detects Hic-5 when phosphorylated at Tyr60. serving as a key tool to study its activation and role in signaling pathways. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to explore Hic-5’s involvement in TGF-β signaling, fibrosis, cancer progression (e.g., epithelial-mesenchymal transition), and mechanotransduction. Its specificity helps distinguish phosphorylated Hic-5 from other LIM proteins or non-phosphorylated isoforms, aiding in understanding how Hic-5 phosphorylation impacts cellular responses to microenvironmental cues. Validation typically includes testing in cell models with known Hic-5 expression and phosphorylation status.
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