| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RBBP8, CTIP, DNA endonuclease RBBP8, RIM, SAE2, COM1, CtBP-interacting protein, JWDS, RBBP-8, SCKL2, Seckel syndrome 2 |
| Entrez GeneID | 5932; |
| WB Predicted band size | 102kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human RBBP8 (Phospho-Ser664) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBBP8 (Phospho-Ser664)抗体的3篇参考文献，涵盖其功能研究和应用场景：
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1. **文献名称**：*Phosphorylation of CtIP at Serine 664 Promotes DNA Repair by Modulating End Resection*
**作者**：Wang et al. (2018)
**摘要**：该研究揭示了CtIP在Ser664位点的磷酸化对DNA双链断裂修复中末端切除的关键作用。通过使用Phospho-Ser664特异性抗体，作者证明ATM/ATR激酶介导的磷酸化增强了CtIP与修复复合物的结合，促进同源重组修复，并利用点突变(S664A)验证了抗体的特异性。
2. **文献名称**：*RBBP8 Phosphorylation Status Predicts Clinical Outcomes in Triple-Negative Breast Cancer*
**作者**：Lee et al. (2020)
**摘要**：本研究通过免疫组化分析乳腺癌样本中RBBP8 (Phospho-Ser664)的表达水平，发现高磷酸化状态与患者化疗敏感性及生存率改善相关。抗体验证显示其在肿瘤组织中的特异性标记能力，为预后生物标志物提供了依据。
3. **文献名称**：*Dynamic Regulation of CtIP Phosphorylation during Cell Cycle Progression*
**作者**：Zhang et al. (2021)
**摘要**：文章利用Phospho-Ser664抗体追踪CtIP在细胞周期中的磷酸化动态变化，发现S664位点在S/G2期显著激活，并与CDK1激酶活性相关。研究通过siRNA敲低和抑制剂实验，证实该修饰对复制应激应答至关重要。
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**注**：以上文献为示例，实际引用需根据具体研究补充完整信息(期刊名称、卷号等)。若需精准文献，建议通过PubMed或Web of Science以关键词“RBBP8 Ser664 phosphorylation antibody”进一步筛选。
The RBBP8 (Phospho-Ser664) antibody detects the phosphorylated form of the RBBP8 protein at serine residue 664. RBBP8. also known as CtIP (CtBP-interacting protein), plays a critical role in DNA double-strand break (DSB) repair, particularly in homologous recombination (HR). Phosphorylation at Ser664 is a key post-translational modification regulated by ATM/ATR kinases in response to DNA damage, facilitating its interaction with repair proteins like BRCA1. This modification is essential for promoting resection of DNA ends to generate single-stranded DNA (ssDNA), a prerequisite for HR-mediated repair.
The antibody is widely used in research to study DNA damage response mechanisms, cell cycle checkpoints, and genomic stability. It helps identify activated RBBP8 in contexts such as ionizing radiation, chemotherapeutic agents, or replication stress. Applications include Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation dynamics in cell lines, tissues, or in vivo models. Validation often involves knockout/knockdown controls or treatment with kinase inhibitors to confirm specificity. Dysregulation of RBBP8 phosphorylation is implicated in cancer progression, therapy resistance, and developmental disorders, making this antibody a valuable tool in oncology and molecular biology studies. Researchers also explore its role in CRISPR-Cas9 gene editing fidelity and as a potential biomarker for DNA repair-deficient cancers.
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