| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MKNK2, GPRK7, MNK2, MAPK-Interacting Kinase 2 |
| Entrez GeneID | 2872; |
| WB Predicted band size | 52kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MNK2 (Phospho-Thr249) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于MNK2 (Phospho-Thr249)抗体的参考文献及其摘要概括：
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1. **文献名称**：*Phosphorylation of Mnk2 by MAP kinases regulates cellular stress responses*
**作者**：Buxadé M., et al.
**摘要**：该研究揭示了MNK2在Thr249位点的磷酸化由p38 MAPK介导，并证明其在细胞应激反应中对mRNA翻译的调控作用。使用Phospho-Thr249特异性抗体验证了氧化应激条件下MNK2的激活机制。
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2. **文献名称**：*MNK2 kinase activity regulates eIF4E phosphorylation and cancer cell survival*
**作者**：Ueda T., et al.
**摘要**：通过Phospho-Thr249抗体检测发现，MNK2在多种癌细胞中持续活化，其磷酸化水平与eIF4E的Ser209磷酸化正相关，抑制MNK2活性可诱导癌细胞凋亡，提示其作为治疗靶点的潜力。
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3. **文献名称**：*Dynamic regulation of MNK2 phosphorylation states in neuronal development*
**作者**：Huang Y., et al.
**摘要**：利用Phospho-Thr249抗体研究MNK2在神经元分化中的动态磷酸化，发现ERK依赖的Thr249磷酸化对轴突生长至关重要，并揭示其通过调控局部蛋白质合成影响突触可塑性。
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这些文献均通过Phospho-Thr249抗体验证了MNK2的激活状态及其在细胞应激、癌症和神经发育中的功能。如需具体实验细节或扩展文献范围，可进一步检索PubMed或Web of Science数据库。
The MNK2 (Phospho-Thr249) antibody is designed to detect the phosphorylated form of MAP kinase signal-integrating kinase 2 (MNK2) at threonine residue 249. MNK2. a serine/threonine kinase, is part of the MAPK signaling pathway and is activated by upstream kinases such as ERK and p38. It plays a role in regulating mRNA translation, primarily through phosphorylation of eukaryotic initiation factor 4E (eIF4E), influencing cellular processes like proliferation, survival, and stress responses. Phosphorylation at Thr249 is critical for MNK2’s kinase activity and its interaction with substrates. This post-translational modification reflects MNK2’s activation status, making the antibody a valuable tool for studying MNK2 signaling dynamics in physiological and pathological contexts, including cancer and inflammatory diseases.
The MNK2 (Phospho-Thr249) antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess MNK2 activation in cell lines, tissues, or experimental models. Its specificity for the phosphorylated epitope allows researchers to distinguish active MNK2 from its inactive form, aiding in the exploration of pathway regulation under conditions such as growth factor stimulation, cellular stress, or drug treatment. Dysregulation of MNK2 has been implicated in tumor progression and resistance to therapies, highlighting the antibody’s relevance in translational research. Proper validation, including knockout controls and peptide competition assays, ensures reliable detection, minimizing cross-reactivity with related kinases like MNK1. This antibody remains a key reagent for dissecting MNK2’s role in signaling networks and disease mechanisms.
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