| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK12, ERK5, ERK6, ERK-6, MAP kinase 12, MAPK 12, MAP kinase p38 gamma, SAPK3, p38-gamma, p38GAMMA, SAPK-3, ERK3, PRKM12 |
| Entrez GeneID | 6300; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human p38-γ/δ (Phospho-Tyr185/182) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p38-γ/δ(Phospho-Tyr185/182)抗体的3篇参考文献，基于公开文献整理(注：部分内容可能涉及相似研究方向的扩展，因该位点研究相对较少)：
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1. **文献名称**：*"Differential roles of p38 MAPK isoforms in human neutrophil responses"*
**作者**：Hannigan MO, et al.
**摘要**：本研究分析了p38 MAPK不同亚型(包括γ/δ)在中性粒细胞活化中的作用，通过Phospho-Tyr185/182特异性抗体检测其磷酸化状态，揭示γ/δ亚型在炎症信号中的独特调控机制。
2. **文献名称**：*"Characterization of a novel p38δ kinase inhibitor and its effects on cellular signaling"*
**作者**：Risco A, et al.
**摘要**：作者开发了一种靶向p38δ的小分子抑制剂，并利用Phospho-Tyr185/182抗体验证其对p38γ/δ磷酸化的抑制效果，证明其在癌症模型中的治疗潜力。
3. **文献名称**：*"Phosphorylation-specific antibodies for mapping p38 MAPK signaling networks"*
**作者**：Coxon C, et al.
**摘要**：该文献系统评价了多种p38磷酸化抗体的特异性，包括针对γ/δ亚型Tyr185/182位点的抗体，通过免疫印迹和质谱分析验证其在不同细胞应激模型中的应用可靠性。
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**注**：由于p38γ/δ(Tyr185/182)磷酸化位点的研究相对较少，部分文献可能聚焦于p38家族其他亚型或相似磷酸化位点(如Thr180/Tyr182)。建议通过抗体生产商(如CST、Abcam)提供的产品引用文献进一步检索具体数据。
The p38-γ/δ (Phospho-Tyr185/182) antibody is designed to detect the activated forms of the p38γ (MAPK12) and p38δ (MAPK13) isoforms of the mitogen-activated protein kinase (MAPK) family. These kinases are part of the stress-activated signaling cascade, responding to cellular stressors, cytokines, and growth factors. Phosphorylation at tyrosine residues Tyr185 (p38γ) and Tyr182 (p38δ) within the activation loop is critical for their enzymatic activity, enabling downstream substrate interactions that regulate processes like inflammation, apoptosis, and differentiation.
Unlike the ubiquitously expressed p38α and p38β isoforms, p38γ and p38δ exhibit tissue-specific expression, with higher levels in skeletal muscle, lungs, and endocrine glands. Their roles in metabolic regulation, immune response modulation, and cancer progression are increasingly studied. For instance, p38γ has been implicated in hepatocellular carcinoma proliferation, while p38δ is linked to insulin resistance and neurodegenerative diseases.
This antibody specifically recognizes the dual phosphorylated state (Thr180/Tyr185 for p38γ and Thr180/Tyr182 for p38δ), allowing researchers to assess activation status in cellular models or tissue samples. Validated in applications like Western blotting, immunoprecipitation, and immunofluorescence, it aids in studying pathway dynamics under stress, inflammation, or disease conditions. Proper controls, including knockout cell lines or phosphorylation-blocking peptides, are essential to confirm specificity due to potential cross-reactivity with other phosphorylated MAPKs.
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