Rabbit Polyclonal Bim(Phospho-Thr56/116) Antibody

The Bim (Phospho-Thr56/116) antibody is designed to detect Bim protein when phosphorylated at threonine residues 56 and 116. key post-translational modifications regulating its pro-apoptotic activity. Bim (Bcl-2-interacting mediator of cell death), a BH3-only member of the Bcl-2 family, promotes apoptosis by neutralizing anti-apoptotic Bcl-2 proteins or directly activating Bax/Bak. Its activity is tightly controlled via phosphorylation-dependent degradation. Phosphorylation at Thr56 (by ERK/MAPK) and Thr116 (by JNK or other stress-activated kinases) triggers ubiquitination and proteasomal degradation, reducing Bim's apoptotic capacity. Conversely, dephosphorylation stabilizes Bim, enhancing cell death under stress. This antibody is widely used to study apoptotic signaling in cancer, neurodegenerative diseases, and immune regulation, particularly in contexts where survival pathways (e.g., RAS/ERK) are hyperactive. It helps assess Bim inactivation mechanisms in therapy resistance or evaluate stress-induced apoptosis in experimental models. Validation often involves treatments with kinase inhibitors (e.g., MEK inhibitors) or apoptotic stimuli to observe phosphorylation changes. Proper controls (e.g., phosphorylation-deficient mutants) are essential to confirm specificity. Understanding Bim phosphorylation dynamics provides insights into cellular fate decisions and therapeutic strategies targeting Bcl-2 family interactions.