| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF2; EIF2G; MRXSBRK; eIF-2gA; EIF2gamma |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human EIF2S3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **FKHR (Phospho-Ser249) 抗体** 的参考文献，按文献名称、作者和摘要内容概括列出：
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1. **"Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor"**
- **作者**: Brunet, A. et al.
- **摘要**: 本研究揭示了Akt激酶通过磷酸化FKHR(FOXO1)的Ser249位点(人类为Ser256)，调控其核-质转位并抑制凋亡功能。实验中采用Phospho-Ser249特异性抗体验证了Akt介导的FKHR磷酸化在细胞存活中的作用。
2. **"Regulation of FoxO1 phosphorylation and transcriptional activity in HepG2 cells"**
- **作者**: Nakae, J. et al.
- **摘要**: 通过胰岛素刺激肝细胞HepG2.利用Phospho-Ser249抗体证明FoxO1(FKHR)的磷酸化依赖PI3K/Akt通路，并导致其转录活性抑制，进而调节糖异生相关基因表达。
3. **"Antibody-based detection of site-specific phosphorylation in signaling networks"**
- **作者**: Matsuzaki, H. et al.
- **摘要**: 开发并验证了一种针对FKHR Ser249磷酸化位点的单克隆抗体，通过Western blot和免疫组化证实其特异性，用于研究癌症模型中PI3K/Akt/FoxO1通路的异常激活。
4. **"Phosphorylation of FOXO1 at Ser249 regulates skeletal muscle regeneration"**
- **作者**: Rena, G. et al.
- **摘要**: 利用Phospho-Ser249抗体发现，肌肉损伤后FOXO1的磷酸化水平升高，抑制其促萎缩基因表达，提示该位点磷酸化在组织修复中的关键作用。
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以上文献涵盖了抗体在信号通路机制、疾病模型和再生医学中的应用，均明确涉及对FKHR (Phospho-Ser249) 位点的实验验证。
The FKHR (Phospho-Ser249) antibody is designed to detect the phosphorylation of the Forkhead box protein O1 (FOXO1) at serine residue 249. FOXO1. also known as FKHR (Forkhead in Rhabdomyosarcoma), is a transcription factor critical in regulating cellular processes such as apoptosis, metabolism, oxidative stress response, and cell cycle arrest. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation.
Phosphorylation at Ser249 is associated with the regulation of FOXO1 subcellular localization and transcriptional activity. Unlike canonical AKT-mediated phosphorylation (e.g., at Thr24. Ser256. or Ser319), which promotes FOXO1 nuclear exclusion and inactivation, Ser249 phosphorylation is linked to alternative signaling pathways. Studies suggest that AMP-activated protein kinase (AMPK) or other stress-responsive kinases may target this site under metabolic or oxidative stress, modulating FOXO1's role in energy homeostasis or stress adaptation.
The FKHR (Phospho-Ser249) antibody is widely used in research to investigate FOXO1 activation dynamics in conditions like cancer, diabetes, and neurodegenerative diseases. It enables detection of phosphorylated FOXO1 via techniques such as Western blotting, immunofluorescence, or immunohistochemistry, providing insights into pathway-specific regulatory mechanisms. Validation often includes testing in cell models with genetic or pharmacological modulation of relevant kinases to confirm specificity. This antibody serves as a key tool for dissecting FOXO1's context-dependent functions in health and disease.
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