| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLDN4, Claudin-4, CPE-receptor, CPETR, CPETR1, Claudin 4, CPE-R, HCPE-R, WBSCR8, CPER |
| Entrez GeneID | 1364; |
| WB Predicted band size | 22kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Claudin 4 (Phospho-Tyr208) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 30% glycerol. |
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以下是三篇关于Claudin 4 (Phospho-Tyr208)抗体的参考文献示例(注：文献为虚拟示例，实际引用需根据真实文献调整)：
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1. **文献名称**: *"Tyrosine phosphorylation of Claudin-4 regulates metastatic potential in breast cancer"*
**作者**: Smith A, et al.
**摘要**: 该研究利用Claudin 4 (Phospho-Tyr208)特异性抗体，发现乳腺癌细胞中Tyr208位点的磷酸化通过破坏紧密连接结构，增强细胞迁移和侵袭能力，提示其作为转移性生物标志物的潜力。
2. **文献名称**: *"Phosphorylation-dependent modulation of intestinal barrier function by Claudin-4"*
**作者**: Lee B, et al.
**摘要**: 通过免疫荧光和Western blot分析，作者使用Phospho-Tyr208抗体证明，炎症因子(如TNF-α)诱导的Claudin 4酪氨酸磷酸化导致肠道上皮屏障通透性增加，与炎症性肠病病理相关。
3. **文献名称**: *"Development and validation of a phospho-specific Claudin-4 antibody for tumor microenvironment studies"*
**作者**: Chen C, et al.
**摘要**: 本研究报道了一种高特异性抗Claudin 4 (Phospho-Tyr208)兔多克隆抗体的开发，验证了其在胰腺癌组织中的临床应用，发现磷酸化水平与患者预后不良显著相关。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词：“Claudin 4 Tyr208 phosphorylation antibody”或结合具体研究领域(如癌症、上皮屏障)进一步筛选。
Claudin-4 is a transmembrane protein belonging to the claudin family, which plays a critical role in forming tight junctions to regulate epithelial and endothelial barrier function. Its phosphorylation at specific tyrosine residues, such as Tyr208. modulates cell-cell adhesion, paracellular permeability, and signaling pathways. Phosphorylation of Tyr208 in Claudin-4 is associated with dynamic remodeling of tight junctions, often influenced by extracellular stimuli or pathological conditions like inflammation and cancer. This post-translational modification may alter interactions with signaling molecules (e.g., MAPK, PI3K/Akt pathways) or cytoskeletal proteins, impacting cell migration, proliferation, and metastatic potential.
Antibodies targeting Claudin-4 (Phospho-Tyr208) are essential tools for studying phosphorylation-dependent mechanisms in physiological and disease contexts. They enable detection of site-specific phosphorylation status via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Research highlights its relevance in cancers (e.g., breast, ovarian, pancreatic), where Claudin-4 overexpression or dysregulated phosphorylation correlates with tumor progression, drug resistance, and poor prognosis. Challenges include ensuring antibody specificity due to potential cross-reactivity with other phosphorylated claudins or epitope similarity. Current studies explore its utility as a biomarker or therapeutic target, particularly in strategies aiming to restore epithelial integrity or inhibit metastasis.
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