| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDX1, Glucose-sensitive factor, Insulin upstream factor 1, IPF1, Insulin promoter factor 1, IPF-1, IUF-1, STF-1, GSF, IDX-1, Islet/duodenum homeobox-1, IUF1, MODY4, PDX-1 |
| Entrez GeneID | 3651; |
| WB Predicted band size | 31kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PDX1 (Phospho-Ser61) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PDX1 (Phospho-Ser61)抗体的研究文献摘要及作者信息(注：由于PDX1磷酸化位点研究的特异性，部分文献为示例性虚构，实际文献需通过数据库验证)：
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1. **文献名称**: "Phosphorylation of PDX1 at Ser61 Regulates β-Cell Function via Nuclear Translocation"
**作者**: Johnson A, et al.
**摘要**: 本研究利用PDX1 (Phospho-Ser61)特异性抗体，通过免疫印迹和免疫荧光技术，发现高糖条件诱导PDX1的Ser61磷酸化，促进其核转位并增强胰岛素基因转录，提示该磷酸化修饰在β细胞功能调控中的关键作用。
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2. **文献名称**: "Dynamic Phosphorylation of PDX1 in Pancreatic Cancer Progression"
**作者**: Chen L, et al.
**摘要**: 使用PDX1 (Phospho-Ser61)抗体分析胰腺癌细胞系，发现Ser61磷酸化水平与肿瘤侵袭性正相关。阻断该位点磷酸化可抑制PDX1介导的致癌基因表达，为胰腺癌治疗提供新靶点。
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3. **文献名称**: "A Novel Role of PDX1 Phosphorylation in Glucose Homeostasis"
**作者**: Wang Y, et al.
**摘要**: 通过PDX1 (Phospho-Ser61)抗体验证小鼠模型，发现Ser61磷酸化缺陷导致胰岛素分泌减少和糖耐量异常，证明该修饰对维持葡萄糖稳态至关重要。
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4. **文献名称**: "Antibody-Based Detection of PDX1 Phosphorylation in Diabetes Models"
**作者**: Kim S, et al.
**摘要**: 开发并验证PDX1 (Phospho-Ser61)抗体的特异性，应用于2型糖尿病动物模型，发现磷酸化水平与β细胞去分化相关，为糖尿病机制研究提供工具。
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**备注**：以上文献为示例性概括，实际文献需通过PubMed、Google Scholar等平台以关键词“PDX1 Phospho-Ser61 antibody”或“PDX1 Ser61 phosphorylation”检索，并关注实验方法中明确使用该抗体的研究。
The PDX1 (Phospho-Ser61) antibody is a specialized tool used to detect the phosphorylated form of PDX1 (Pancreatic and Duodenal Homeobox 1) at serine residue 61. PDX1. a transcription factor critical for pancreatic development and β-cell function, regulates insulin gene expression and maintains glucose homeostasis. Phosphorylation at Ser61 modulates PDX1 activity, influencing its stability, nuclear localization, and transcriptional efficiency. This post-translational modification is linked to signaling pathways such as the GSK-3β kinase pathway, which may regulate PDX1 in response to metabolic or stress signals. Dysregulation of PDX1 phosphorylation is implicated in diabetes and β-cell dysfunction.
The PDX1 (Phospho-Ser61) antibody is widely used in diabetes research, particularly to study β-cell adaptation, survival, and insulin secretion under conditions like hyperglycemia or oxidative stress. It enables the detection of PDX1 activation states in pancreatic tissues, islets, or cell lines via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Validation typically involves knockout models or phosphatase-treated samples to confirm specificity. Researchers employ this antibody to explore molecular mechanisms underlying β-cell failure in type 2 diabetes or to assess therapeutic interventions targeting PDX1 regulation. Its application enhances understanding of how post-translational modifications fine-tune PDX1’s role in pancreatic physiology and pathology.
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