| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SHC3, Neuronal Shc, NSHC, RAI, SH2 domain protein C3, SHC-transforming protein C, N-Shc, p52 and p64 isoforms of n-shc, Protein Rai, SHC-transforming protein 3, SHCC |
| Entrez GeneID | 53358; |
| WB Predicted band size | 64kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Shc3 (Phospho-Tyr424) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Shc3 (Phospho-Tyr424)抗体的3篇示例参考文献(内容为虚构，仅作格式参考)：
1. **文献名称**：*Phosphorylation of Shc3 at Tyr424 Mediates EGFR Signaling in Glioblastoma*
**作者**：Smith A, et al.
**摘要**：本研究揭示了Shc3在EGFR激活的胶质母细胞瘤中的磷酸化调控。通过使用Shc3 (Phospho-Tyr424)抗体的Western blot和免疫荧光实验，发现Tyr424位点的磷酸化促进Shc3与Grb2的结合，驱动下游MAPK通路，增强肿瘤细胞增殖。
2. **文献名称**：*Role of Shc3 Tyrosine Phosphorylation in Neuronal Differentiation*
**作者**：Chen L, et al.
**摘要**：文章利用Shc3 (Phospho-Tyr424)抗体检测神经元分化过程中Tyr424磷酸化水平的变化，证实该修饰通过调节TrkA受体信号传导影响神经突生长，为Shc3在神经发育中的作用提供机制证据。
3. **文献名称**：*Shc3 Phosphorylation at Tyr424 Promotes Breast Cancer Metastasis via FAK Activation*
**作者**：Wang Y, et al.
**摘要**：研究发现Shc3的Tyr424磷酸化在乳腺癌转移中至关重要。通过免疫沉淀结合Shc3 (Phospho-Tyr424)抗体，证实磷酸化Shc3与FAK相互作用，激活侵袭相关通路，为靶向治疗提供新靶点。
(注：以上文献为示例，实际研究中请通过PubMed或Google Scholar以关键词“Shc3 Tyr424 phosphorylation”或抗体货号检索相关论文。)
The SHC3 (Phospho-Tyr424) antibody is designed to detect the phosphorylated form of SHC3 at tyrosine residue 424. SHC3. a member of the SHC (Src homology 2 domain-containing) family of adaptor proteins, plays a critical role in intracellular signaling by linking activated receptor tyrosine kinases (RTKs) to downstream pathways like RAS/MAPK. Tyrosine phosphorylation at specific sites, such as Tyr424. is essential for SHC3’s function, enabling its interaction with signaling intermediates (e.g., GRB2) to propagate growth, differentiation, or survival signals. This phosphorylation event typically occurs in response to extracellular stimuli, including growth factors or cytokines, and is tightly regulated by tyrosine kinases and phosphatases.
The SHC3 (Phospho-Tyr424) antibody is widely used in research to study SHC3 activation status in cellular signaling contexts. Its applications include Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation-dependent SHC3 involvement in physiological or pathological processes. Dysregulation of SHC3 signaling has been implicated in cancers and neurological disorders, making this antibody a valuable tool for investigating disease mechanisms. Specificity validation—via knockout controls or phosphopeptide competition—is crucial to ensure accurate detection, as SHC isoforms share structural homology. Researchers employ this antibody to explore SHC3's role in RTK-driven pathways, particularly in neural or cancer cell models, where Tyr424 phosphorylation may correlate with disease progression or therapeutic resistance.
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