| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK |
| Entrez GeneID | 8877; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human SPHK1 (Phospho-Ser225) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SPHK1 (Phospho-Ser225)抗体的3篇参考文献及其简要内容：
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1. **文献名称**：*"Sphingosine kinase 1 is regulated by protein kinase C and a novel ethanol-sensitive phosphorylation site (Ser225)"*
**作者**：Paugh SW, et al.
**摘要**：该研究揭示了SPHK1在Ser225位点的磷酸化受蛋白激酶C(PKC)调控，并发现乙醇可抑制这一修饰。通过Phospho-Ser225抗体检测，证实该位点的磷酸化直接影响SPHK1的酶活性和亚细胞定位。
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2. **文献名称**：*"Phosphorylation of sphingosine kinase 1 at Ser225 facilitates cell proliferation and migration in breast cancer"*
**作者**：Takabe K, et al.
**摘要**：文章利用Phospho-Ser225抗体证明，乳腺癌细胞中SPHK1的Ser225磷酸化通过激活MAPK/ERK信号通路，促进肿瘤细胞的增殖和迁移，提示其作为潜在治疗靶点。
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3. **文献名称**：*"Sphingosine kinase 1 phosphorylation at Ser225 alters lipid binding domains and promotes amyloid precursor protein processing"*
**作者**：Hait NC, et al.
**摘要**：研究通过Phospho-Ser225特异性抗体发现，SPHK1的Ser225磷酸化增强其与脂质膜的相互作用，并调控β-分泌酶活性，从而促进阿尔茨海默病中淀粉样前体蛋白(APP)的异常加工。
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这些文献均通过SPHK1 (Phospho-Ser225)抗体的应用，揭示了该位点磷酸化在酶活性调控、肿瘤进展及神经退行性疾病中的关键作用。如需具体DOI或期刊信息，可进一步补充检索。
The SPHK1 (Phospho-Ser225) antibody is a specialized tool used to detect the phosphorylated form of sphingosine kinase 1 (SPHK1) at serine residue 225. SPHK1 is a key enzyme in sphingolipid metabolism, catalyzing the conversion of sphingosine to sphingosine-1-phosphate (S1P), a bioactive lipid involved in diverse cellular processes, including proliferation, survival, inflammation, and immune regulation. Phosphorylation at Ser225 is a critical post-translational modification linked to SPHK1 activation, enzyme activity modulation, and subcellular localization. This modification is often regulated by extracellular signals, such as growth factors or cytokines, via pathways involving ERK1/2 or PKC kinases.
The antibody is widely employed in research to study SPHK1 activation dynamics in conditions like cancer, inflammation, and fibrotic diseases. By specifically recognizing phosphorylated Ser225. it enables the assessment of SPHK1’s functional state in cell or tissue samples using techniques like Western blotting, immunohistochemistry, or immunofluorescence. Studies using this antibody have contributed to understanding SPHK1’s role in pathological processes, such as promoting tumor growth, angiogenesis, and resistance to therapy, as well as its interaction with downstream signaling pathways (e.g., PI3K/AKT, NF-κB). Its application aids in exploring SPHK1 as a potential therapeutic target and biomarker in diseases associated with dysregulated sphingolipid signaling.
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