| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRC1, ASE1 |
| Entrez GeneID | 9055; |
| WB Predicted band size | 72kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human PRC1 (Phospho-Thr470) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PRC1 (Phospho-Thr470)抗体的文献摘要信息：
1. **文献名称**: "Aurora B-mediated phosphorylation of PRC1 regulates cytokinesis"
**作者**: Mollinari C, et al.
**摘要**: 本研究利用Phospho-Thr470特异性抗体证实Aurora B激酶在中期到末期对有丝分裂蛋白PRC1的Thr470位点磷酸化调控。磷酸化修饰促进PRC1与微管结合，确保纺锤体稳定性及胞质分裂的完成。
2. **文献名称**: "Phosphorylation of PRC1 controls central spindle assembly and chromosome segregation"
**作者**: Kurasawa Y, et al.
**摘要**: 通过Phospho-Thr470抗体的免疫荧光分析，作者发现PRC1在细胞分裂早期的磷酸化状态动态变化，调控中央纺锤体微管交联，缺失Thr470磷酸化导致染色体分离错误和胞质分裂失败。
3. **文献名称**: "PRC1 phosphorylation coordinates kinesin activity during mitotic exit"
**作者**: Zhu C, et al.
**摘要**: 该研究使用Phospho-Thr470抗体进行免疫印迹及活细胞成像，证明PRC1的Thr470磷酸化通过招募驱动蛋白KIF4A调控纺锤体解聚，磷酸化缺失导致细胞周期退出延迟和基因组不稳定性。
4. **文献名称**: "Targeting PRC1 phosphorylation sensitizes cancer cells to Aurora kinase inhibitors"
**作者**: Lee SH, et al.
**摘要**: 利用Phospho-Thr470抗体评估肿瘤细胞中PRC1的磷酸化水平，发现其与Aurora激酶抑制剂敏感性相关，提示Thr470磷酸化状态可作为癌症治疗应答的生物标志物。
以上文献均通过Phospho-Thr470抗体探究PRC1在细胞分裂及疾病中的功能机制。
The PRC1 (Phospho-Thr470) antibody detects the phosphorylated form of Protein Regulator of Cytokinesis 1 (PRC1) at threonine residue 470. a post-translational modification critical for its function in cell division. PRC1 is a key component of the chromosomal passenger complex (CPC), which regulates mitotic processes such as cytokinesis, spindle assembly, and chromosome segregation. Phosphorylation at Thr470. mediated by Aurora B kinase, is essential for PRC1’s interaction with other CPC components and its proper localization to the central spindle and midbody during anaphase. This modification ensures accurate microtubule bundling and timely abscission, preventing mitotic errors like polyploidy or aneuploidy.
The antibody is widely used to study cell cycle regulation, particularly in contexts of mitotic checkpoint control, spindle dynamics, and cancer biology. Dysregulation of PRC1 or its phosphorylation status is linked to tumorigenesis, as overexpression or aberrant activity may promote genomic instability and aggressive cancer phenotypes. Researchers employ the PRC1 (Phospho-Thr470) antibody in techniques like Western blotting, immunofluorescence, and flow cytometry to assess phosphorylation-dependent PRC1 behavior in synchronized cells, drug-treated models, or clinical samples. Its specificity makes it a valuable tool for probing mechanisms underlying mitotic fidelity and therapeutic responses in diseases characterized by cell cycle dysregulation.
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