| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EPHB3, Cek10, ETK2, HEK2 protein kinase, Embryonic kinase 2, EPH receptor B3, EPH-like kinase 2, Ephrin type-B receptor 3, MDK5, Sek4, TYRO6, EK2, EPH-like tyrosine kinase 2, EPH-like tyrosine kinase-2, HEK2, Human embryo kinase 2, Tyrosine-protein k ... |
| Entrez GeneID | 2049; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human EPHB3 (Phospho-Tyr608) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 30% glycerol. |
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以下是关于 **EPHB3 (Phospho-Tyr608) 抗体** 的模拟参考文献示例(文献为虚构，仅供格式参考)：
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1. **文献名称**：*"EPHB3 tyrosine phosphorylation regulates cell migration in colorectal cancer"*
**作者**：Zhang L, et al.
**摘要**：研究利用抗 EPHB3 (Phospho-Tyr608) 抗体验证了 EPHB3 受体在结直肠癌细胞中的自磷酸化现象，并发现 Tyr608 位点的磷酸化通过激活 Rho-GTPase 信号通路促进肿瘤细胞迁移。
2. **文献名称**：*"Structural basis of EPHB3 activation by ligand-induced phosphorylation at Tyr608"*
**作者**：Kim S, et al.
**摘要**：通过 X 射线晶体学和磷酸化特异性抗体检测，揭示了 Ephrin-B 配体结合诱导 EPHB3 受体 Tyr608 位点磷酸化的分子机制，并阐明其介导细胞间排斥信号的构象变化。
3. **文献名称**：*"A phospho-specific antibody screen identifies EPHB3 as a key regulator of synaptic plasticity"*
**作者**：Wang Y, et al.
**摘要**：利用抗 EPHB3 (Phospho-Tyr608) 抗体进行脑组织免疫组化分析，发现 Tyr608 磷酸化水平在海马区突触可塑性中显著上调，提示 EPHB3 可能参与学习记忆的分子调控。
4. **文献名称**：*"Validation of a novel EPHB3 (pTyr608) antibody for immunohistochemical applications"*
**作者**：Garcia R, et al.
**摘要**：研究报道了一种高特异性 EPHB3 (Phospho-Tyr608) 抗体的开发与验证，证实其在 Western blot 和免疫荧光中可灵敏检测肿瘤组织样本中的磷酸化 EPHB3 活性形式。
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注：以上文献为示例性内容，实际研究中请参考真实发表的学术论文(可通过 PubMed 或 Google Scholar 检索关键词如 "EPHB3 phosphorylation Tyr608")。
The EPHB3 (Phospho-Tyr608) antibody detects the phosphorylated form of tyrosine 608 (Y608) in the Ephrin type-B receptor 3 (EPHB3), a member of the Eph receptor tyrosine kinase family. EPHB3 plays critical roles in cell-cell communication, regulating processes like cell adhesion, migration, and tissue boundary formation. Upon binding to ephrin-B ligands, Eph receptors undergo dimerization and autophosphorylation at specific tyrosine residues, including Y608 in the kinase domain. This phosphorylation event activates downstream signaling pathways, such as MAPK/ERK and PI3K/AKT, which influence cellular responses in development, homeostasis, and disease.
The EPHB3 (Phospho-Tyr608) antibody is a tool to study the activation status of EPHB3. as phosphorylation at Y608 is indicative of receptor engagement and signaling activity. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate EPHB3’s role in cancer (e.g., colorectal, breast), neuronal development, and angiogenesis. Dysregulation of EPHB3 phosphorylation is linked to tumor progression, metastasis, and altered cell polarity.
Validated for specificity, this antibody helps researchers explore context-dependent signaling mechanisms and therapeutic targets. Proper sample preparation, including phosphatase inhibition, is essential to preserve phosphorylation status during experiments.
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