Rabbit Polyclonal 14-3-3σ(Phospho-Ser186) Antibody

The 14-3-3σ (Phospho-Ser186) antibody is a specialized tool for detecting the phosphorylated form of the 14-3-3σ protein at serine residue 186. The 14-3-3σ isoform, also known as stratifin or HME1. is a member of the conserved 14-3-3 protein family, which regulates diverse cellular processes, including cell cycle progression, apoptosis, and stress responses. This isoform is encoded by the *SFN* gene and is predominantly expressed in epithelial cells, where it acts as a tumor suppressor by binding to and sequestering cyclin-dependent kinases (CDKs) or pro-survival factors, thereby promoting cell cycle arrest or apoptosis under stress conditions. Phosphorylation at Ser186 is a critical post-translational modification that modulates 14-3-3σ’s activity, stability, or interactions with partner proteins. This modification may be induced by DNA damage or other cellular stresses, potentially through kinases such as ATM/ATR or casein kinase 2 (CK2). The 14-3-3σ (Phospho-Ser186) antibody is widely used in research to study DNA damage response pathways, cell cycle checkpoints, and cancer biology, particularly in contexts where 14-3-3σ dysfunction contributes to tumorigenesis or chemoresistance. Its specificity enables detection of the phosphorylated protein in techniques like Western blotting, immunofluorescence, or immunohistochemistry, aiding mechanistic insights into stress signaling and therapeutic targeting.