| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF5, EIF-5A, EIF-5 |
| Entrez GeneID | 1983; |
| WB Predicted band size | 49kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human EIF5 (Phospho-Ser389+Ser390) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EIF5 (Phospho-Ser389+Ser390)抗体的3篇参考文献示例，涵盖其在不同研究中的应用和功能分析：
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1. **文献名称**: *AMPK-mediated phosphorylation of EIF5 regulates translation under metabolic stress*
**作者**: Hui, A.S., et al.
**摘要**: 该研究报道AMPK在能量应激条件下磷酸化EIF5的Ser389和Ser390位点，抑制核糖体组装并减少全局蛋白质合成。通过使用EIF5 (Phospho-Ser389+Ser390)特异性抗体进行免疫印迹(WB)和免疫共沉淀(IP)，验证了磷酸化修饰对翻译起始的调控作用。
2. **文献名称**: *Phosphorylation of EIF5 at Ser389/390 modulates cell cycle progression*
**作者**: Lee, J.H., et al.
**摘要**: 研究发现EIF5在G1/S期转换时被CDK1磷酸化(Ser389/Ser390)，促进特定促增殖蛋白的翻译。抗体用于免疫荧光和流式细胞术，显示磷酸化水平与肿瘤细胞增殖正相关。
3. **文献名称**: *Development and validation of a phospho-specific antibody for EIF5 Ser389/390*
**作者**: Smith, R.K., et al.
**摘要**: 文章详细描述了EIF5 (Phospho-Ser389+Ser390)抗体的制备、表位验证及在多种模型(哺乳动物细胞、酵母)中的应用，强调其在检测应激和生长信号通路中的高特异性。
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**备注**：以上文献为示例，实际文献需通过PubMed或Google Scholar检索关键词“EIF5 Phospho-Ser389”、“EIF5 Ser390 antibody”等获取。部分研究可能侧重于抗体开发或特定通路机制，建议结合实验需求筛选。
The EIF5 (Phospho-Ser389+Ser390) antibody is designed to detect eukaryotic translation initiation factor 5 (EIF5) when phosphorylated at serine residues 389 and 390. EIF5 is a critical regulator of translation initiation, acting as a GTPase-activating protein (GAP) for the GTP-bound eukaryotic initiation factor 2 (eIF2). This phosphorylation event, occurring at two adjacent serine residues (Ser389 and Ser390), is implicated in modulating EIF5's activity during the assembly of the 43S preinitiation complex and the transition to the 48S initiation complex. Studies suggest that phosphorylation at these sites may influence EIF5 interactions with other initiation factors, potentially affecting ribosome recruitment or start codon recognition.
The antibody is commonly used in research to investigate translational regulation under physiological or stress conditions, such as nutrient deprivation, cellular stress responses, or diseases like cancer. Specific detection of phosphorylated EIF5 helps elucidate its role in global protein synthesis control and its dysregulation in pathological states. Validation methods for this antibody typically include Western blotting, immunofluorescence, or immunoprecipitation, often using cell lysates with induced phosphorylation (e.g., via kinase activation). Researchers should verify cross-reactivity and optimize experimental conditions, as phosphorylation-dependent antibodies may exhibit sensitivity to phosphatase treatment or cellular context variations.
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