| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MYD88, MYD88D |
| Entrez GeneID | 4615; |
| WB Predicted band size | 34kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Myd88 (Phospho-Tyr257) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Myd88 (Phospho-Tyr257)抗体的3篇参考文献，按研究背景和内容筛选整理：
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1. **文献名称**：*MyD88 tyrosine phosphorylation is critical for TLR/IL-1R signaling and host defense*
**作者**：Janssens S, et al.
**摘要**：该研究首次报道MyD88在Tyr257位点的磷酸化对TLR/IL-1R信号通路至关重要，通过构建磷酸化缺陷突变体证明其影响下游NF-κB激活及炎症因子释放，并开发特异性抗pTyr257抗体用于功能验证。
2. **文献名称**：*Phosphorylation-dependent regulation of MyD88 innate immune function*
**作者**：Chen K, et al.
**摘要**：本文利用Phospho-Tyr257特异性抗体，通过免疫沉淀和质谱分析揭示MyD88在LPS刺激下的酪氨酸磷酸化动态变化，证明该修饰促进其与IRAK4的相互作用，并增强巨噬细胞的抗菌反应。
3. **文献名称**：*Targeting MyD88 phosphorylation in autoimmune diseases: Insights from antibody-based detection*
**作者**：Li X, et al.
**摘要**：研究团队开发了一种高灵敏度的抗MyD88 pTyr257单克隆抗体，应用于类风湿性关节炎患者样本检测，发现磷酸化MyD88水平与疾病活动度正相关，提示其作为治疗靶点的潜力。
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**备注**：上述文献为模拟示例，实际引用需通过PubMed或Web of Science等平台检索具体论文。若该位点研究较少，可扩展至MyD88磷酸化整体机制或相关信号通路的研究。
The Myd88 (Myeloid Differentiation Primary Response 88) protein is a critical adaptor molecule in innate immune signaling, primarily mediating signals from Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). Upon receptor activation, Myd88 recruits downstream kinases like IRAKs, triggering NF-κB and MAPK pathways to induce pro-inflammatory cytokines. Phosphorylation at Tyr257 is a key regulatory event for Myd88 activation, influencing its oligomerization, interaction with downstream effectors, and overall signaling efficiency.
The Myd88 (Phospho-Tyr257) antibody is a specialized tool designed to detect Myd88 when phosphorylated at tyrosine 257. This post-translational modification is associated with active signaling states, making the antibody valuable for studying TLR/IL-1R pathway activation in immune responses, inflammation, and diseases like cancer or autoimmune disorders. Researchers commonly use it in techniques such as Western blotting, immunofluorescence, or immunoprecipitation to assess Myd88 activation dynamics. Its specificity for the phosphorylated form helps distinguish basal Myd88 levels from its active, signaling-competent state. Validating this antibody typically involves testing in cell models with induced TLR/IL-1R stimulation or tyrosine kinase inhibitors to confirm phosphorylation-dependent reactivity. Understanding Myd88 phosphorylation provides insights into dysregulated immune pathways and potential therapeutic targets.
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