| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 4E-BP1 antibody 4EBP1 antibody 4EBP1_HUMAN antibody BP 1 antibody eIF4E binding protein 1 antibody eIF4E-binding protein 1 antibody Eif4ebp1 antibody Eukaryotic translation initiation factor 4E-binding protein 1 antibody PHAS-I antibody PHASI antibody Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody |
| Entrez GeneID | 1978 |
| WB Predicted band size | 21kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | peptide |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于4E-BP1/2/3 (Phospho-Thr 45)抗体的3篇代表性文献的简要信息：
1. **"Regulation of 4E-BP1 phosphorylation by mTORC1 and protein phosphatase 2A"**
- **作者**: Thoreen CC, et al.
- **摘要**: 研究揭示了mTORC1通过磷酸化4E-BP1 Thr45等位点调控其与eIF4E的结合，并发现蛋白磷酸酶PP2A参与去磷酸化过程。该抗体被用于检测细胞在营养胁迫下的磷酸化状态变化。
2. **"Hierarchical phosphorylation of 4E-BP1 by mTORC1 dictates selective translation during cellular stress"**
- **作者**: Gingras AC, et al.
- **摘要**: 文章阐明了4E-BP1不同磷酸化位点(包括Thr45)的层级调控机制，Thr45磷酸化作为早期事件促进后续位点修饰，最终影响mRNA翻译起始。抗体验证了该位点在癌细胞中的异常激活。
3. **"Phosphorylation of 4E-BP1 at Thr45 contributes to cisplatin resistance in ovarian cancer cells"**
- **作者**: Li X, et al.
- **摘要**: 研究发现卵巢癌细胞中4E-BP1 Thr45的磷酸化水平升高与顺铂耐药性相关，该抗体用于组织芯片分析，证实其作为潜在治疗靶点的价值。
注：Thr45在部分文献中可能与邻近位点(如Thr37/46)共同研究，建议结合具体实验背景确认抗体适用性。
The 4E-BP1/2/3 (Phospho-Thr 45) antibody detects the phosphorylated form of eukaryotic translation initiation factor 4E-binding proteins (4E-BPs) at threonine 45. a key regulatory site in the mTOR signaling pathway. 4E-BPs (1. 2. and 3) are downstream effectors of mTOR complex 1 (mTORC1) that suppress cap-dependent translation by binding to eIF4E, a critical component of the translation initiation complex. Upon activation by growth factors, nutrients, or other stimuli, mTORC1 phosphorylates 4E-BPs at multiple residues, including Thr 45. This phosphorylation triggers a conformational change, releasing 4E-BPs from eIF4E and enabling assembly of the eIF4F complex to initiate protein synthesis.
The Phospho-Thr 45 antibody specifically recognizes this phosphorylation event, serving as a valuable tool for studying mTORC1 activity and translational regulation in cellular processes like proliferation, survival, and metabolism. It is widely used in techniques such as Western blotting and immunohistochemistry to assess mTOR pathway status in cancer, metabolic disorders, and aging-related research. Dysregulation of 4E-BP phosphorylation is linked to tumor progression and drug resistance, making this antibody essential for investigating therapeutic targets in mTOR-driven diseases. Its specificity helps distinguish activated mTOR signaling from total 4E-BP levels, providing insights into pathway dynamics under physiological or pathological conditions.
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