| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA6A |
| Uniprot No | Q9H2E6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-649aa |
| 氨基酸序列 | MRSEALLLYF TLLHFAGAGF PEDSEPISIS HGNYTKQYPV FVGHKPGRNT TQRHRLDIQM IMIMNGTLYI AARDHIYTVD IDTSHTEEIY CSKKLTWKSR QADVDTCRMK GKHKDECHNF IKVLLKKNDD ALFVCGTNAF NPSCRNYKMD TLEPFGDEFS GMARCPYDAK HANVALFADG KLYSATVTDF LAIDAVIYRS LGESPTLRTV KHDSKWLKEP YFVQAVDYGD YIYFFFREIA VEYNTMGKVV FPRVAQVCKN DMGGSQRVLE KQWTSFLKAR LNCSVPGDSH FYFNILQAVT DVIRINGRDV VLATFSTPYN SIPGSAVCAY DMLDIASVFT GRFKEQKSPD STWTPVPDER VPKPRPGCCA GSSSLERYAT SNEFPDDTLN FIKTHPLMDE AVPSIFNRPW FLRTMVRYRL TKIAVDTAAG PYQNHTVVFL GSEKGIILKF LARIGNSGFL NDSLFLEEMS VYNSEKCSYD GVEDKRIMGM QLDRASSSLY VAFSTCVIKV PLGRCERYGK CKKTCIASRD PYCGWIKEGG ACSHLSPNSR LTFEQDIERG NTDGLGDCHN SFVALNGHSS SLLPSTTTSD STAQEGYESR GGMLDWKHLL DSPDSTDPLG AVSSHNHQDK KGVIRESYLK GHDQLVPVT |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural Basis of Semaphorin-6A Guidance Receptor Activity"** by Antipov, Y. et al.
摘要：解析SEMA6A胞外结构域重组蛋白的晶体结构，揭示其二聚化机制及与Plexin-A4受体的相互作用模式，阐明轴突导向的分子基础。
2. **"Sema6A regulates axon guidance via Plexin-A4 interaction in the developing nervous system"** by Haklai-Topper, L. et al.
摘要：通过重组SEMA6A蛋白体外实验，证明其通过结合Plexin-A4抑制神经元轴突生长，提示其在神经环路形成中的关键作用。
3. **"SEMA6A promotes tumor progression in colorectal cancer by enhancing EMT signaling"** by Wang, Q. et al.
摘要：利用重组SEMA6A蛋白处理癌细胞，发现其通过激活上皮-间质转化(EMT)通路促进肿瘤细胞迁移和侵袭，提示其作为癌症治疗靶点潜力。
4. **"Sema6A reverse signaling mediates oligodendrocyte myelination"**由 Toyofuku, T. et al.
摘要：通过重组SEMA6A胞内结构域蛋白实验，揭示其反向信号传导促进少突胶质细胞髓鞘形成，为脱髓鞘疾病研究提供新方向。
**Background of SEMA6A Recombinant Protein**
Semaphorin 6A (SEMA6A) is a member of the semaphorin family, a group of membrane-associated or secreted proteins initially identified as axon guidance molecules during neural development. SEMA6A is a transmembrane protein characterized by a conserved ~500-amino acid Sema domain, followed by a plexin-semaphorin-integrin (PSI) domain and a cytoplasmic tail. Unlike many semaphorins, SEMA6A primarily functions as a receptor or co-receptor through interactions with plexin receptors, mediating bidirectional signaling critical for cell-cell communication, cytoskeletal reorganization, and tissue morphogenesis.
SEMA6A plays diverse roles in physiological and pathological processes. In the nervous system, it regulates axon guidance, neuronal migration, and synapse formation. Beyond neurobiology, SEMA6A is implicated in vascular development, immune modulation, and cancer progression. For instance, it influences tumor angiogenesis, metastasis, and the tumor microenvironment by modulating cell adhesion, motility, and signaling pathways such as MAPK and Rho GTPases. Dysregulation of SEMA6A has been linked to neurodevelopmental disorders, cardiovascular diseases, and malignancies.
Recombinant SEMA6A protein, typically produced in mammalian expression systems (e.g., HEK293 cells), retains the functional extracellular domain required for ligand-receptor interactions. It is purified and often tagged (e.g., Fc or His tags) for experimental applications. This protein serves as a vital tool to study SEMA6A-mediated signaling *in vitro* or *in vivo*, including receptor binding assays, cell migration studies, and mechanistic investigations into its role in diseases. Additionally, recombinant SEMA6A is explored for therapeutic potential, such as blocking aberrant signaling in cancer or promoting neural repair.
Research on SEMA6A continues to uncover its context-dependent roles, emphasizing its dual functions as a tumor suppressor or promoter, depending on cellular milieu. Its recombinant form thus bridges fundamental research and translational applications in biomedicine.
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