| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | S100A3 |
| Uniprot No | P33764 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-101aa |
| 氨基酸序列 | MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于S100A3重组蛋白的3篇参考文献示例(内容基于公开研究整理，非真实文献)：
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1. **文献名称**: *"Crystal structure of human S100A3 reveals unique calcium-dependent structural changes"*
**作者**: Kizawa K., et al.
**摘要**: 该研究解析了重组人源S100A3蛋白的晶体结构，揭示了其钙离子结合后的构象变化，并讨论了其与靶蛋白(如角蛋白)相互作用的潜在机制。
2. **文献名称**: *"Expression and purification of recombinant S100A3 in E. coli: implications for hair follicle biology"*
**作者**: Fujimoto Y., et al.
**摘要**: 文章优化了S100A3在大肠杆菌中的重组表达和纯化方法，并通过体外实验证明该蛋白在毛囊细胞分化中可能通过调控钙信号通路发挥作用。
3. **文献名称**: *"S100A3 promotes keratinocyte migration via RAGE signaling in psoriasis-like inflammation"*
**作者**: Eckert R.L., et al.
**摘要**: 研究利用重组S100A3蛋白发现其通过结合RAGE受体激活下游炎症通路，促进银屑病模型中角质形成细胞的迁移，为皮肤病治疗提供新靶点。
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注：以上文献为示例，实际引用时请通过PubMed或学术数据库检索真实存在的论文。
**Background of S100A3 Recombinant Protein**
The S100A3 protein is a member of the S100 family, a group of calcium-binding proteins characterized by two EF-hand structural motifs. Primarily expressed in humans, S100A3 is noted for its unique tissue-specific expression, with high levels detected in hair follicles, particularly in trichocytes (hair cuticle cells), suggesting a role in keratinocyte differentiation and hair shaft formation. Unlike other S100 proteins, S100A3 exhibits an unusually high affinity for zinc, which modulates its calcium-binding properties and influences its intracellular functions.
Structurally, S100A3 exists as a homodimer, interacting with target proteins in a calcium-dependent manner to regulate cellular processes such as cytoskeletal dynamics, cell cycle progression, and signal transduction. Its expression is linked to epithelial tissues, and dysregulation has been associated with pathological conditions, including psoriasis, certain skin cancers, and alopecia. Studies also suggest its potential role in tumorigenesis, though findings remain controversial, with both pro- and anti-tumor effects reported depending on context.
Recombinant S100A3 is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, structure-function relationships, and interactions. Purified via affinity chromatography, the recombinant protein is utilized in *in vitro* assays, antibody development, and mechanistic studies to elucidate its physiological and pathological roles. Research on S100A3 continues to explore its diagnostic or therapeutic potential, particularly in dermatological and oncological contexts.
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