| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARCI9; LASS3 |
| Entrez GeneID | 204219; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide corresponding to a region derived from internal residues of human ceramide synthase 3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及CERS3抗体的研究文献及简要摘要：
1. **"Ceramide synthase 3 regulates skin barrier homeostasis"**
作者：Eckhardt et al.
摘要：研究利用CERS3抗体在小鼠模型中检测表皮层中CERS3蛋白的表达，发现其缺失导致皮肤屏障功能障碍，证明CERS3对鞘脂代谢和角质细胞分化至关重要。
2. **"CERS3 deficiency disrupts sperm development and causes male infertility"**
作者：Zhou et al.
摘要：通过CERS3抗体的免疫荧光实验，发现CERS3在精子形成中高表达，其缺失导致精子形态异常和雄性不育，揭示CERS3在生殖系统中的鞘脂合成作用。
3. **"Role of ceramide synthase 3 in nervous system myelination"**
作者：Pewzner-Jung et al.
摘要：使用CERS3抗体的Western blot和免疫组化分析显示，CERS3在中枢神经系统髓鞘形成中表达，其缺陷导致小鼠运动功能障碍和髓鞘结构异常，提示其在神经保护中的关键作用。
注：以上文献信息为示例性概括，实际文献可能存在差异。建议通过PubMed或Google Scholar以“CERS3 antibody”为关键词检索最新研究。
CERS3 (ceramide synthase 3) is a key enzyme in sphingolipid metabolism, responsible for catalyzing the synthesis of ultra-long-chain ceramides (C≥28) by acylating sphinganine with specific very long-chain fatty acids. These ceramides are critical components of the epidermal lipid barrier, maintaining skin hydration and permeability. Dysregulation of CERS3 is linked to inherited skin disorders like autosomal recessive congenital ichthyosis (ARCI), where mutations in the CERS3 gene disrupt epidermal differentiation and barrier integrity.
CERS3 antibodies are essential tools for studying its expression, localization, and function in skin biology and disease models. They enable detection of CERS3 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate its role in lipid raft formation, cellular signaling, and keratinocyte differentiation. Research using these antibodies has highlighted tissue-specific expression patterns, with high levels in the skin, testes, and brain.
Recent studies also explore CERS3's potential beyond dermatology, including roles in neuronal development and cancer, where altered sphingolipid metabolism may influence tumor progression. Validated CERS3 antibodies (polyclonal/monoclonal) are crucial for translational research, offering insights into therapeutic strategies targeting sphingolipid pathways. However, challenges remain in ensuring antibody specificity due to homology among ceramide synthase isoforms.
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