Rabbit Polyclonal CEP70 Antibody

The CEP70 antibody is a research tool targeting Centrosomal Protein 70 (CEP70), a key component of the centrosome, a microtubule-organizing structure critical for cell division and cytoskeletal regulation. CEP70 is a ~70 kDa protein localized to the pericentriolar matrix and plays a role in centrosome maturation, spindle formation, and chromosomal segregation during mitosis. Dysregulation of CEP70 has been linked to mitotic errors, genomic instability, and diseases such as cancer, ciliopathies, and neurodevelopmental disorders. CEP70 antibodies are primarily used in immunofluorescence, Western blotting, and immunoprecipitation to study centrosome dynamics, cell cycle progression, and disease mechanisms. They help visualize CEP70's spatial-temporal localization during mitosis, assess its interaction with other centrosomal proteins (e.g., γ-tubulin, pericentrin), and evaluate its aberrant expression in pathological contexts. Recent studies suggest CEP70 overexpression in certain cancers correlates with poor prognosis and chemoresistance, while its deficiency is implicated in microcephaly and ciliary defects. Commercial CEP70 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes testing in knockout cell lines or siRNA-treated samples to confirm specificity. Ongoing research aims to clarify CEP70's role in centriole duplication, cilia assembly, and its potential as a therapeutic target.