| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGBL5 |
| Uniprot No | Q8NDL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-724aa |
| 氨基酸序列 | MNMNKQSKLYSQGMAPFVRTLPTRPRWERIRDRPTFEMTETQFVLSFVHRFVEGRGATTFFAFCYPFSYSDCQELLNQLDQRFPENHPTHSSPLDTIYYHRELLCYSLDGLRVDLLTITSCHGLREDREPRLEQLFPDTSTPRPFRFAGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHYRTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSRLNSQSSSEHQPSSCLPPDAPVSDLEKANNLQNEAQCGHSADRHNAEAWKQTEPAEQKLNSVWIMPQQSAGLEESAPDTIPPKESGVAYYVDLHGHASKRGCFMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSEHSSLTNLRAWMLKHVRNSRGLSSTLNVGVNKKRGLRTPPKSHNGLPVSCSENTLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPAGLPGLGSSTQKVTHRVLGPVREPRSQDRRRQQQPLNHRPAGSLAPSPAPTSSGPASSHKLGSCLLPDSFNIPGSSCSLLSSGDKPEAVMVIGKGLLGTGARMPCIKTRLQTCPRRVSARRGPGFPRLGPGWAGAHRRLAEG |
| 分子量 | 106.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人胞质羧肽酶样蛋白5(AGBL5)的示例参考文献(内容为虚构，仅供格式参考)：
1. **文献名称**：AGBL5介导的微管蛋白去酪氨酸化在神经发育中的功能
**作者**：Smith J, et al.
**摘要**：该研究揭示了AGBL5作为胞质羧肽酶，通过特异性切除α-微管蛋白的C端酪氨酸残基调控神经元轴突生长，其缺失导致小鼠突触可塑性和运动协调能力异常。
2. **文献名称**：AGBL5在胶质瘤中的表观遗传沉默与肿瘤侵袭性关联
**作者**：Wang L, et al.
**摘要**：研究发现AGBL5在胶质母细胞瘤中因启动子高甲基化而表达下调，其缺失通过破坏细胞骨架动态性促进肿瘤迁移，恢复表达可抑制体内外侵袭表型。
3. **文献名称**：人AGBL5的晶体结构揭示其催化机制
**作者**：Zhang Y, et al.
**摘要**：通过X射线衍射解析AGBL5的三维结构，证实其锌离子依赖性催化中心及底物结合口袋特征，为设计靶向抑制剂提供了结构基础。
4. **文献名称**：AGBL5敲除小鼠模型揭示其在胚胎血管生成中的作用
**作者**：Johnson R, et al.
**摘要**：研究利用条件性敲除小鼠模型，发现AGBL5通过调节血管内皮细胞微管动力学影响胚胎期血管分支，缺陷导致胎盘发育异常及胚胎致死。
(注：以上文献均为示例，实际文献需通过PubMed、Web of Science等平台检索。)
**Background of AGBL5 (Cytoplasmic Carboxypeptidase-like Protein 5)**
AGBL5. also known as ATP/GTP Binding Protein-like 5. is a member of the metallocarboxypeptidase family. It belongs to the ATP/GTP-binding protein (AGBL) subfamily, which shares structural similarities with carboxypeptidases but often lacks canonical enzymatic activity. AGBL5 is primarily localized in the cytoplasm and is implicated in post-translational protein modifications, particularly the removal of glutamic acid residues from α-tubulin and other substrates. This process, termed deglutamylation, regulates microtubule dynamics, influencing cellular processes like mitosis, intracellular transport, and neuronal development.
Structurally, AGBL5 contains a conserved carboxypeptidase domain with a zinc-binding active site, though catalytic activity varies among homologs. Studies suggest it may act as a regulatory protein, modulating substrate specificity or collaborating with other enzymes. Research highlights its role in neurobiology, as abnormal tubulin modification is linked to neurodegenerative disorders. AGBL5 is also studied in cancer due to altered expression in tumors, such as glioblastoma, where it may affect cell proliferation and invasion.
Despite these insights, AGBL5's precise mechanisms and physiological roles remain under investigation. Current work focuses on its interactions with cytoskeletal components, metabolic pathways, and potential involvement in hormone processing (e.g., thyroid hormone inactivation). Further studies are needed to elucidate its therapeutic potential in disease contexts.
×
