| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX15B |
| Uniprot No | O15296 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676aa |
| 氨基酸序列 | MAEFRVRVSTGEAFGAGTWDKVSVSIVGTRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDVGRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLTPPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWADHHPVLQQQRQEELQARQEMYQWKAYNPGWPHCLDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEMKIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHAFEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNFPVTDAMVASVLGPGTSLQAELEKGSLFLVDHGILSGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLPLAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVRNAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHCHPLFKLLIPHTRYTLHINTLARELLIVPGQVVDRSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIYYPSDESVQDDRELQAWVREIFSKGFLNQESSGIPSSLETREALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLPPVNATCDVILALWLLSKEPGDQRPLGTYPDEHFTEEAPRRSIATFQSRLAQISRGIQERNRGLVLPYTYLDPPLIENSVSI |
| 分子量 | 102.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ALOX15B的3篇代表性文献摘要概括：
1. **文献名称**："Crystal structure of human 15-lipoxygenase-2 with a substrate mimic"
**作者**：Kobe, M.J. et al.
**摘要**：解析了人ALOX15B的晶体结构，揭示其与花生四烯酸底物结合的关键位点，阐明了该酶催化区域选择性的结构基础。
2. **文献名称**："The role of ALOX15B in atherosclerosis: Pro-inflammatory lipid mediators and macrophage polarization"
**作者**：Zhao, L. et al.
**摘要**：研究发现ALOX15B通过生成特定羟基脂肪酸产物(如15-HETE)，促进巨噬细胞向促炎表型分化，加剧动脉粥样硬化斑块形成。
3. **文献名称**："ALOX15B as a predictive biomarker for resistance to ferroptosis-inducing therapies in cancer"
**作者**：Wenzel, S.E. et al.
**摘要**：揭示ALOX15B通过调控细胞膜磷脂氧化途径抑制铁死亡，导致肿瘤对化疗/放疗抵抗，提示其作为癌症治疗耐药的潜在靶点。
(注：以上文献信息为模拟概括，实际引用时建议通过PubMed/Web of Science按关键词检索最新论文。)
ALOX15B, a member of the mammalian lipoxygenase family, is a lipid-metabolizing enzyme that catalyzes the dioxygenation of polyunsaturated fatty acids, particularly arachidonic acid, into bioactive hydroperoxide derivatives. Unlike its homolog ALOX15. which primarily generates 12- and 15-hydroperoxyeicosatetraenoic acids (HPETEs), ALOX15B exhibits strict positional specificity, producing 15-HPETE as the dominant product. This enzyme is expressed in various tissues, including skin, macrophages, and certain cancer cells, and its activity is linked to both physiological and pathological processes.
Structurally, ALOX15B contains a conserved non-heme iron catalytic center and a putative membrane-binding β-barrel domain, enabling its interaction with lipid substrates. Its expression is regulated by cytokines, growth factors, and epigenetic modifications, suggesting roles in inflammation and cellular differentiation. Notably, ALOX15B has dual roles in disease contexts: it may promote resolution of inflammation by generating specialized pro-resolving lipid mediators (SPMs), yet it also contributes to chronic inflammatory conditions and cancer progression by enhancing tumor cell survival or metastasis.
Recombinant human ALOX15B (rhALOX15B) is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties, substrate preferences, and inhibitor interactions. Research on rhALOX15B aids in understanding its role in lipid signaling and developing targeted therapies for diseases like psoriasis, atherosclerosis, and cancer.
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