| 纯度 | >90%SDS-PAGE. |
| 种属 | Arabidopsis thaliana |
| 靶点 | ANAC |
| Uniprot No | Q9C932 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MGIQETDPLT QLSLPPGFRF YPTDEELMVQ YLCRKAAGYD FSLQLIAEID LYKFDPWVLP NKALFGEKEW YFFSPRDRKY PNGSRPNRVA GSGYWKATGT DKIISTEGQR VGIKKALVFY IGKAPKGTKT NWIMHEYRLI EPSRRNGSTK LDDWVLCRIY KKQSSAQKQV YDNGIANARE FSNNGTSSTT SSSSHFEDVL DSFHQEIDNR NFQFSNPNRI SSLRPDLTEQ KTGFHGLADT SNFDWASFAG NVEHNNSVPE LGMSHVVPNL EYNCGYLKTE EEVESSHGFN NSGELAQKGY GVDSFGYSGQ VGGFGFM |
| 分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"The Arabidopsis NAC transcription factor ANAC019 links stress responses to flavonol biosynthesis"**
- **作者**: Fennell, H., et al.
- **摘要**: 研究发现NAC019在拟南芥中通过激活黄酮醇合成通路基因(如CHS和FLS1)的表达，参与非生物胁迫(如干旱和氧化应激)的响应，同时黄酮醇积累增强植物抗氧化能力。
2. **"NAC019 interacts with ABI5 to regulate seed germination and seedling growth in Arabidopsis"**
- **作者**: Jiang, Y., et al.
- **摘要**: NAC019与ABA信号通路关键因子ABI5直接互作，共同调控种子萌发和幼苗生长。过量表达NAC019导致ABA敏感性增强，抑制萌发，而突变体表现出对ABA的耐受性。
3. **"The RING-H2 type E3 ligase SINAT5 regulates the stability of NAC019 during drought stress"**
- **作者**: Meng, X., et al.
- **摘要**: NAC019的蛋白稳定性受E3泛素连接酶SINAT5调控。干旱胁迫下SINAT5介导NAC019的泛素化降解，负调控其抗旱功能，揭示转录后水平对NAC019活性的精细调控。
4. **"Overexpression of ANAC019 improves drought tolerance in transgenic Arabidopsis by regulating stomatal closure and root architecture"**
- **作者**: Kim, S.G., et al.
- **摘要**: 过量表达NAC019的拟南芥通过促进气孔关闭和根系结构重塑(如增加侧根)增强抗旱性，同时上调胁迫响应基因(如DREB2A和RD29B)。
NAC transcription factors, named after the petunia NAM, Arabidopsis ATAF1/2. and CUC2/3 proteins, are plant-specific regulators involved in diverse biological processes. Arabidopsis NAC019. a member of this family, contains a conserved N-terminal NAC DNA-binding domain and a variable C-terminal transcriptional activation/repression domain. Initially identified as a stress-responsive gene, NAC019 is induced by abiotic stresses such as drought, high salinity, and abscisic acid (ABA). It functions as a transcriptional activator in ABA-mediated signaling pathways, regulating downstream stress-responsive genes like ERD1. Studies using transgenic plants reveal that NAC019 overexpression enhances drought tolerance but may accelerate senescence under non-stress conditions, suggesting a trade-off between stress resistance and growth. Its activity is modulated by interactions with other regulators, including the RING-type E3 ligase SINAT5 for proteasomal degradation. While NAC019 shares functional redundancy with NAC055 and NAC072 in stress responses, its specific role in the ABA-dependent regulatory network distinguishes it from other NAC members. Current research focuses on unraveling its target genes, post-translational modifications, and potential applications in improving crop stress resilience. However, balancing its stress-adaptive benefits with growth penalties remains a challenge for biotechnological utilization.
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