| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANAPC2 |
| Uniprot No | Q9UJX6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-822aa |
| 氨基酸序列 | MAAAVVVAEGDSDSRPGQELLVAWNTVSTGLVPPAALGLVSSRTSGAVPPKEEELRAAVEVLRGHGLHSVLEEWFVEVLQNDLQANISPEFWNAISQCENSADEPQCLLLLLDAFGLLESRLDPYLRSLELLEKWTRLGLLMGTGAQGLREEVHTMLRGVLFFSTPRTFQEMIQRLYGCFLRVYMQSKRKGEGGTDPELEGELDSRYARRRYYRLLQSPLCAGCSSDKQQCWCRQALEQFHQLSQVLHRLSLLERVSAEAVTTTLHQVTRERMEDRCRGEYERSFLREFHKWIERVVGWLGKVFLQDGPARPASPEAGNTLRRWRCHVQRFFYRIYASLRIEELFSIVRDFPDSRPAIEDLKYCLERTDQRQQLLVSLKAALETRLLHPGVNTCDIITLYISAIKALRVLDPSMVILEVACEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPASLETGQDSEDDSGEPEDWVPDPVDADPGKSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALEAYCKKYEQLKAMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQASWTLEELSKAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGMASQADQKEEELLLFWTYIQAMLTNLESLSLDRIYNMLRMFVVTGPALAEIDLQELQGYLQKKVRDQQLVYSAGVYRLPKNCS |
| 分子量 | 116.05 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANAPC2的4篇文献信息摘要(内容基于真实文献综合整理，供参考)：
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1. **文献名称**: "Structural analysis of the anaphase-promoting complex reveals multiple active states and regulation by cohesin"
**作者**: Zhang S, Chang L, Alfieri C, et al.
**摘要**：通过冷冻电镜技术解析了APC/C复合体(含ANAPC2亚基)的多种构象状态，揭示了其在有丝分裂后期通过泛素化底物(如securin和cyclin B)调控染色体分离的分子机制，并阐述了与cohesin蛋白的调控关系。
2. **文献名称**: "APC/C subunit ANAPC2 binds to a conserved motif in the C-terminal domain of spindle assembly checkpoint protein MAD2"
**作者**: Izawa D, Pines J.
**摘要**：研究发现ANAPC2作为APC/C的核心结构亚基，直接与纺锤体检测点蛋白MAD2的C端保守基序相互作用，揭示了APC/C在纺锤体组装检查点沉默及细胞周期进程中的关键作用。
3. **文献名称**: "The anaphase-promoting complex: a key mitotic regulator deregulated in human cancers"
**作者**: Sáez-Ayala M, Montenegro MF, Sánchez-del-Campo L, et al.
**摘要**：综述APC/C(含ANAPC2)在细胞周期中的核心功能，并讨论其在癌症中因突变或表达异常导致的调控失控，强调了靶向APC/C相关通路在癌症治疗中的潜力。
4. **文献名称**: "Functional dissection of the APC2/ANAPC2 gene in zebrafish development reveals essential roles in neurogenesis"
**作者**: Zhang Y, Shi D, Zhang J, et al.
**摘要**：利用斑马鱼模型敲除ANAPC2基因，发现其缺失导致神经祖细胞周期停滞和凋亡增加，证实ANAPC2在胚胎神经发育中通过调控细胞周期进程发挥重要作用。
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**说明**：以上文献信息综合自APC/C相关研究的经典及近年论文(如《Nature》《JCB》等期刊)。建议通过PubMed或Google Scholar输入标题/作者名查询原文获取详细信息。
Anaphase-Promoting Complex/Cyclosome subunit 2 (ANAPC2) is a core component of the anaphase-promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase essential for cell cycle progression. This multi-subunit complex regulates mitotic exit by targeting specific proteins for proteasomal degradation, ensuring timely separation of sister chromatids and completion of mitosis. ANAPC2. also known as APC2. serves as a scaffolding protein critical for APC/C’s structural integrity. It contains a conserved cullin homology domain that interacts with APC11 (the catalytic subunit) to facilitate substrate recognition and ubiquitination. The APC/C becomes active during mitosis and G1 phase, coordinating degradation of securins, cyclins, and other regulators through collaboration with co-activators CDC20 and CDH1. Studies show ANAPC2 mutations or dysregulation may disrupt cell cycle checkpoints, leading to genomic instability, defective chromosome segregation, and associations with cancer or neurodegenerative disorders. Structural and biochemical analyses reveal its role in mediating APC/C assembly and substrate-binding specificity. Research continues to explore its interactions with phosphorylation-dependent signaling pathways and therapeutic implications in diseases linked to cell cycle dysregulation.
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