| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARF4L |
| Uniprot No | P49703 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-201aa |
| 氨基酸序列 | GNHLTEMAP TASSFLPHFQ ALHVVVIGLD SAGKTSLLYR LKFKEFVQSV PTKGFNTEKI RVPLGGSRGI TFQVWDVGGQ EKLRPLWRSY TRRTDGLVFV VDAAEAERLE EAKVELHRIS RASDNQGVPV LVLANKQDQP GALSAAEVEK RLAVRELAAA TLTHVQGCSA VDGLGLQQGL ERLYEMILKR KKAARGGKKR R |
| 分子量 | 22.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人腺苷二磷酸核糖基化因子样蛋白4D(ARL4D)的示例性参考文献，涵盖其功能、机制及相关疾病研究。请注意，以下内容为基于典型研究方向的假设性示例，实际文献需通过学术数据库进一步确认：
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1. **文献名称**: *ARL4D promotes hepatocellular carcinoma progression via activating Wnt/β-catenin signaling*
**作者**: Jiang Y, et al. (2020)
**摘要**: 该研究揭示了ARL4D在肝细胞癌(HCC)中显著高表达，并通过激活Wnt/β-catenin信号通路促进肿瘤细胞增殖、迁移及侵袭，提示其作为HCC预后标志物和治疗靶点的潜力。
2. **文献名称**: *ARL4D mediates cytoskeletal remodeling in macrophage migration through ARF6 interaction*
**作者**: Li X, et al. (2021)
**摘要**: 本文报道ARL4D通过结合ARF6 GTP酶，调控巨噬细胞中肌动蛋白骨架重组，影响细胞趋化性和炎症反应，为免疫微环境中的细胞运动机制提供新见解。
3. **文献名称**: *Crystal structure and GTPase activity analysis of human ARL4D*
**作者**: Wang T, et al. (2018)
**摘要**: 通过解析ARL4D的晶体结构，阐明其GTP结合特性及催化机制，发现其与效应蛋白的结合位点差异，为靶向ARL家族蛋白的药物设计奠定基础。
4. **文献名称**: *ARL4D regulates neuronal migration and cortical development in mice*
**作者**: Chen L, et al. (2022)
**摘要**: 研究表明，ARL4D缺失导致小鼠大脑皮质神经元迁移障碍和层状结构异常，提示其在神经发育疾病中的潜在作用。
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**建议**：以上为示例，实际文献请通过PubMed、Google Scholar等平台以关键词“ARL4D/ARL家族蛋白/细胞迁移”等检索，或查阅《Journal of Cell Science》《Oncogene》《Cell Communication and Signaling》等相关期刊。
ARL4D (adenine nucleotide-binding protein ADP-ribosylation factor-like 4D), a member of the ARF/ARL small GTPase family, plays critical roles in membrane trafficking, cytoskeleton reorganization, and cellular signaling. It shares structural homology with other ARL proteins but exhibits distinct features, such as the absence of a myristoylation site, influencing its subcellular localization. Functionally, ARL4D interacts with effector molecules like cytohesins and participates in modulating actin dynamics, vesicle transport, and cell migration. Studies highlight its involvement in cellular processes linked to development, including neuronal differentiation and immune regulation. Dysregulation of ARL4D has been associated with pathologies such as cancers and developmental disorders, emphasizing its potential as a therapeutic target. Recombinant ARL4D, produced via expression systems like *E. coli* or mammalian cells, enables mechanistic studies by providing purified protein for structural analysis, interaction assays, and functional investigations. Its recombinant form is particularly valuable for elucidating GTP/GDP cycling kinetics, post-translational modifications, and binding partners, advancing our understanding of ARL4D’s role in health and disease. Additionally, recombinant ARL4D serves as a tool for screening small-molecule modulators, aiding drug discovery efforts targeting GTPase-mediated pathways.
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