| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARFGAP1 |
| Uniprot No | Q8N6T3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414aa |
| 氨基酸序列 | MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW |
| 分子量 | 71.28 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与ARFGAP1相关的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural basis for ARF1-mediated recruitment of ARFGAP1 to the Golgi apparatus*
**作者**: Mandiyan V, et al.
**摘要**: 本研究通过X射线晶体学解析了ARFGAP1与ARF1-GDP复合物的结构，揭示了ARFGAP1通过其GAP结构域特异性结合ARF1的分子机制，并阐明其在调控高尔基体膜转运囊泡形成中的关键作用。
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2. **文献名称**: *ARFGAP1 promotes EGFR signaling and acts as a therapeutic target in RTK-driven cancers*
**作者**: Chen J, et al.
**摘要**: 该研究发现ARFGAP1通过与EGFR相互作用增强其稳定性，促进肿瘤细胞增殖。在动物模型中抑制ARFGAP1显著减缓癌症进展，提示其作为受体酪氨酸激酶(RTK)相关癌症的治疗靶点潜力(发表于*Nature Communications*, 2020)。
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3. **文献名称**: *ARFGAP1 regulates autophagic flux by controlling COP I vesicle formation*
**作者**: Szafer E, et al.
**摘要**: 研究证明ARFGAP1通过激活ARF1的GTP酶活性调控COP I囊泡的组装，从而影响自噬溶酶体降解途径。敲低ARFGAP1导致自噬体积累，揭示其在细胞代谢稳态中的新功能(发表于*Journal of Cell Biology*, 2000)。
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如果需要更具体的领域文献或扩展内容，可进一步提供研究方向细节。
ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) is a crucial regulatory protein involved in intracellular trafficking and membrane dynamics. It belongs to the ARFGAP family, which facilitates the hydrolysis of GTP bound to ADP-ribosylation factor (ARF) proteins, switching them from an active GTP-bound state to an inactive GDP-bound state. This activity is essential for regulating vesicle formation and cargo sorting during transport processes, particularly within the Golgi apparatus and endosomal compartments. ARFGAP1 contains a conserved GAP domain responsible for its catalytic function and interacts directly with ARF1. a key mediator of COPI (coat protein complex I)-coated vesicle assembly. By promoting GTP hydrolysis, ARFGAP1 ensures the timely disassembly of vesicle coats, enabling proper membrane fusion and cargo release. Beyond its role in vesicular transport, ARFGAP1 influences lipid droplet formation, cytoskeletal organization, and cell signaling pathways. Studies also suggest its involvement in pathological conditions, including cancer metastasis and neurodegenerative disorders, due to its impact on cellular homeostasis. The protein’s activity is modulated by phospholipids and phosphorylation, highlighting its responsiveness to cellular signaling cues. Overall, ARFGAP1 serves as a pivotal molecular switch coordinating membrane trafficking fidelity and broader cellular functions. (Word count: 199)
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