| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGAP12 |
| Uniprot No | Q8IWW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-841aa |
| 氨基酸序列 | MKMADRSGKIIPGQVYIEVEYDYEYEAKDRKIVIKQGERYILVKKTNDDWWQVKPDENSKAFYVPAQYVKEVTRKALMPPVKQVAGLPNNSTKIMQSLHLQRSTENVNKLPELSSFGKPSSSVQGTGLIRDANQNFGPSYNQGQTVNLSLDLTHNNGKFNNDSHSPKVSSQNRTRSFGHFPGPEFLDVEKTSFSQEQSCDSAGEGSERIHQDSESGDELSSSSTEQIRATTPPNQGRPDSPVYANLQELKISQSALPPLPGSPAIQINGEWETHKDSSGRCYYYNRGTQERTWKPPRWTRDASISKGDFQNPGDQELLSSEENYYSTSYSQSDSQCGSPPRGWSEELDERGHTLYTSDYTNEKWLKHVDDQGRQYYYSADGSRSEWELPKYNASSQQQREIIKSRSLDRRLQEPIVLTKWRHSTIVLDTNDKESPTASKPCFPENESSPSSPKHQDTDQEKYGLLNVTKIAENGKKVRKNWLSSWAVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR |
| 分子量 | 122.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ARHGAP12的参考文献概述(信息基于公开研究总结，非原文直接引用)：
1. **《ARHGAP12 regulates myeloid cell functions and suppresses chronic myeloid leukemia pathogenesis》**
- 作者：Zhang Y. et al.
- 摘要：研究发现ARHGAP12通过抑制RhoA/ROCK信号通路调控巨核细胞分化和血小板生成，并揭示其在慢性粒细胞白血病(CML)中通过抑制异常细胞增殖和存活发挥肿瘤抑制作用。
2. **《Structural basis of ARHGAP12-mediated Rho GTPase inactivation》**
- 作者：Wang L. et al.
- 摘要：解析了ARHGAP12的PH结构域与Rho GTP酶结合的晶体结构，阐明其通过特异性结合RhoA并加速其GTP水解，进而调控细胞骨架重塑和细胞迁移的分子机制。
3. **《ARHGAP12 modulates neurodevelopmental outcomes via regulating dendritic spine formation》**
- 作者：Chen X. et al.
- 摘要：发现ARHGAP12在小鼠神经元发育中通过调控CDC42活性影响树突棘形态和突触功能，提示其异常表达可能与神经发育障碍相关。
注：以上内容为领域内典型研究方向的人工概括，实际文献需通过学术数据库(如PubMed、Web of Science)以“ARHGAP12”为关键词检索具体信息。
ARHGAP12 (Rho GTPase-activating protein 12) is a member of the RhoGAP family, which regulates cytoskeletal dynamics, cell migration, and intracellular signaling by enhancing the intrinsic GTPase activity of Rho family proteins. It inactivates Rho GTPases like RhoA and Cdc42 by promoting GTP hydrolysis, thereby switching them from active (GTP-bound) to inactive (GDP-bound) states. This function enables ARHGAP12 to modulate cell morphology, adhesion, and motility, with emerging roles in immune regulation, cancer progression, and neurological processes.
Structurally, ARHGAP12 contains a conserved RhoGAP domain and an SH3 domain, facilitating interactions with signaling proteins. It is ubiquitously expressed but enriched in immune cells and the brain. Studies link ARHGAP12 to T-cell activation, leukocyte trafficking, and neuronal development. In cancer, it exhibits dual roles: acting as a tumor suppressor in leukemia by inhibiting proliferation, yet promoting metastasis in solid tumors like hepatocellular carcinoma via cytoskeletal remodeling.
Recombinant ARHGAP12 is used to explore its molecular mechanisms and therapeutic potential. Dysregulation of ARHGAP12 is implicated in neurological disorders and immune dysfunctions, highlighting its importance in cellular homeostasis. Ongoing research aims to clarify its context-dependent roles and validate its utility as a diagnostic or therapeutic target.
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