| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARGLU1 |
| Uniprot No | Q9NWB6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-273aa |
| 氨基酸序列 | MGRSRSRSSSRSKHTKSSKHNKKRSRSRSRSRDKERVRKRSKSRESKRNRRRESRSRSRSTNTAVSRRERDRERASSPPDRIDIFGRTVSKRSSLDEKQKREEEEKKAEFERQRKIRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEELERQRQAELAAQKAREVTLGRLESRDSPWQNFQCWVLPPAQFRKRWNTDYLIPFSSKLNIAAKVNFLAYSEVLTDNLKVGSFYKTYSRILFDLMELAI |
| 分子量 | 59.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人精氨酸和谷氨酸丰富蛋白1(**ARGLU1**)的3篇参考文献的简要整理：
1. **"ARGLU1 interacts with TRA2B to regulate alternative splicing in cell differentiation"**
- **作者**：Smith J, et al.
- **摘要**：研究发现ARGLU1通过与剪接因子TRA2B的直接结合，调控靶基因的可变剪接，影响胚胎干细胞的神经分化过程。敲除ARGLU1导致小鼠胚胎干细胞分化异常，提示其在发育中的关键作用。
2. **"ARGLU1 modulates transcriptional coactivation via interaction with steroid receptors"**
- **作者**：Kim S, Lee M.
- **摘要**：该文献揭示ARGLU1作为核受体(如雌激素受体)的转录共激活因子，通过富含精氨酸/谷氨酸的结构域招募组蛋白乙酰转移酶p300.增强靶基因的转录活性，影响激素依赖性癌症进展。
3. **"ARGLU1 deficiency disrupts synaptic plasticity and cognitive function in mice"**
- **作者**：Garcia-R, et al.
- **摘要**：在小鼠模型中，ARGLU1敲除导致海马突触可塑性受损及学习记忆障碍，其机制与ARGLU1调控突触相关RNA结合蛋白(如FMRP)的表达和剪接有关，提示其在神经疾病中的潜在病理作用。
*注：上述文献信息为模拟示例，需通过学术数据库(如PubMed/Web of Science)确认真实研究。建议检索时结合关键词“ARGLU1 function”或“ARGLU1 splicing/transcription”提高效率。*
Arginine and Glutamic Acid-Rich Protein 1 (ARGLU1), also known as Coiled-Coil Domain-Containing Protein 34 (CCDC34), is a nuclear protein encoded in humans by the *ARGLU1* gene. It is characterized by its high content of arginine (R) and glutamic acid (E) residues, forming intrinsically disordered regions (IDRs) that facilitate dynamic protein interactions. ARGLU1 plays critical roles in transcriptional regulation and RNA splicing, interacting with the RNA polymerase II complex and spliceosome components to modulate gene expression. Studies suggest its involvement in resolving RNA:DNA hybrids (R-loops), thereby maintaining genome stability and regulating alternative splicing events.
Functionally, ARGLU1 has been linked to developmental processes, including neural and immune system maturation. Its dysregulation is implicated in diseases such as cancer, where altered expression correlates with tumor progression and metastasis, possibly through aberrant splicing of oncogenic transcripts. Structural studies highlight its conserved coiled-coil domains, which mediate interactions with transcriptional coactivators like SERTAD1. enhancing transcriptional activation.
ARGLU1’s dynamic, phase-separation-prone regions align with its role in forming nuclear condensates, suggesting a mechanism for spatial organization of transcriptional machinery. Despite progress, its precise molecular pathways remain under investigation, particularly in context-specific roles across tissues and diseases. This protein represents a promising target for understanding epigenetic regulation and therapeutic intervention in splicing-related disorders.
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