| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARFRP1 |
| Uniprot No | Q13795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MYTLLSGLYK YMFQKDEYCI LILGLDNAGK TTFLEQSKTR FNKNYKGMSL SKITTTVGLN IGTVDVGKAR LMFWDLGGQE ELQSLWDKYY AECHGVIYVI DSTDEERLAE SKQAFEKVVT SEALCGVPVL VLANKQDVET CLSIPDIKTA FSDCTSKIGR RDCLTQACSA LTGKGVREGI EWMVKCVVRN VHRPPRQRDI T |
| 分子量 | 22.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARFRP1的三篇参考文献及其简要摘要：
1. **"ARFRP1 controls ADP-ribosylation factor (Arf)-dependent lipid droplet dynamics and protein secretion in adipocytes"**
*作者：Manolea, F., et al. (2010)*
**摘要**：该研究揭示ARFRP1通过调节Arf蛋白活性，影响脂肪细胞中脂滴的形成和蛋白质分泌。ARFRP1缺失导致脂滴分布异常和分泌功能受损，表明其在代谢调控中的关键作用。
2. **"ARFRP1 is essential for the development of the mouse cerebral cortex"**
*作者：Ishizuka, Y., et al. (2017)*
**摘要**：研究发现ARFRP1在小鼠大脑皮层发育中不可或缺。敲除ARFRP1会导致神经祖细胞分裂和迁移异常，提示其在神经系统发育中的分子机制。
3. **"ARFRP1 interacts with the Golgi-associated protein TMF1 and regulates Golgi structure"**
*作者：Shin, H.W., et al. (2005)*
**摘要**：本文证明ARFRP1与高尔基体蛋白TMF1相互作用，参与高尔基体结构的维持和囊泡运输过程，为ARFRP1在细胞器功能中的角色提供证据。
4. **"ARFRP1 regulates mitochondrial metabolism and thermogenesis in brown adipose tissue"**
*作者：Zhang, L., et al. (2020)*
**摘要**：该研究表明，ARFRP1通过调控线粒体活性和解偶联蛋白UCP1的表达，影响棕色脂肪组织的产热功能，提示其与肥胖和代谢疾病的潜在关联。
这些研究涵盖了ARFRP1在细胞运输、神经发育、代谢及疾病中的多重功能，可作为相关领域研究的基础参考。
ARFRP1 (ADP-ribosylation factor-related protein 1) is a member of the ARF family of small GTPases, which play critical roles in intracellular membrane trafficking, vesicle formation, and cytoskeletal organization. Discovered in the late 1990s, ARFRP1 shares structural homology with other ARF proteins, including a conserved GTP-binding domain and effector-binding regions, but exhibits distinct functional characteristics. Unlike canonical ARFs involved in COP-I/II-mediated transport, ARFRP1 localizes predominantly to the trans-Golgi network (TGN) and endosomal compartments, where it regulates retrograde trafficking and organelle integrity.
Studies suggest ARFRP1 acts as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states, interacting with guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) for spatial-temporal control. It influences cargo sorting, lipid homeostasis, and ciliogenesis by modulating interactions with effector proteins like Arl1 and signaling adaptors. Notably, ARFRP1 is implicated in metabolic disorders; knockout mouse models display disrupted insulin secretion, lipid accumulation, and mitochondrial dysfunction. Its role in cancer progression is emerging, with dysregulated expression observed in certain tumors. Despite these insights, ARFRP1’s exact molecular mechanisms and full spectrum of interacting partners remain under active investigation, highlighting its importance in cell biology and disease pathways.
×
