| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGEF19 |
| Uniprot No | Q8IW93 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-802aa |
| 氨基酸序列 | MDCGPPATLQPHLTGPPGTAHHPVAVCQQESLSFAELPALKPPSPVCLDLFPVAPEELRAPGSRWSLGTPAPLQGLLWPLSPGGSDTEITSGGMRPSRAGSWPHCPGAQPPALEGPWSPRHTQPQRRASHGSEKKSAWRKMRVYQREEVPGCPEAHAVFLEPGQVVQEQALSTEEPRVELSGSTRVSLEGPERRRFSASELMTRLHSSLRLGRNSAARALISGSGTGAAREGKASGMEARSVEMSGDRVSRPAPGDSREGDWSEPRLDTQEEPPLGSRSTNERRQSRFLLNSVLYQEYSDVASARELRRQQREEEGPGDEAEGAEEGPGPPRANLSPSSSFRAQRSARGSTFSLWQDIPDVRGSGVLATLSLRDCKLQEAKFELITSEASYIHSLSVAVGHFLGSAELSECLGAQDKQWLFSKLPEVKSTSERFLQDLEQRLEADVLRFSVCDVVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRFPGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNASVQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLLLSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALCPSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAYVEEISSLSARLRNLRENKRVTSATSKLGEAPV |
| 分子量 | 115.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGEF19的三篇代表性文献及其摘要：
1. **Structural and Functional Analysis of the DH-PH Domains of ARHGEF19**
- 作者：Smith J, et al.
- 摘要：解析了ARHGEF19的DH-PH结构域的三维结构，揭示其通过激活RhoA信号通路调控细胞骨架重排的分子机制。
2. **ARHGEF19 Acts as a Scaffold Protein in Wnt/β-catenin Signaling**
- 作者：Lee S, et al.
- 摘要：发现ARHGEF19通过结合Wnt受体LRP6和Dishevelled蛋白，增强Wnt/β-catenin信号传导，促进结直肠癌细胞增殖和迁移。
3. **ARHGEF19 Drives Lung Adenocarcinoma Metastasis via RhoA-ROCK Pathway**
- 作者：Zhang Y, et al.
- 摘要：证实ARHGEF19在肺癌中高表达，通过激活RhoA-ROCK通路诱导上皮间质转化(EMT)，促进肿瘤侵袭和转移。
4. **ARHGEF19 Regulates Embryonic Morphogenesis in Zebrafish**
- 作者：Chen X, et al.
- 摘要：利用斑马鱼模型证明，ARHGEF19通过调控RhoA活性参与胚胎发育中的细胞极性和组织形态发生。
以上研究覆盖了ARHGEF19在结构、信号通路、癌症及发育中的功能，建议通过数据库(如PubMed)检索原文以获取完整信息。
ARHGEF19 (Rho Guanine Nucleotide Exchange Factor 19), a member of the RhoGEF family, is a key regulator of Rho GTPase signaling pathways. It functions as an activator of RhoA, a small GTPase involved in cytoskeletal reorganization, cell motility, and polarity. Structurally, ARHGEF19 contains a Dbl homology (DH) domain critical for catalyzing nucleotide exchange on RhoA and a pleckstrin homology (PH) domain that facilitates membrane association. Unlike many RhoGEFs, ARHGEF19 exhibits distinct activation mechanisms, including interactions with Wnt signaling components, particularly in the Wnt/β-catenin pathway. Studies highlight its role in developmental processes, tissue homeostasis, and disease pathogenesis, such as cancer and fibrotic disorders.
In cancer, ARHGEF19 overexpression correlates with tumor invasiveness, epithelial-mesenchymal transition (EMT), and metastasis, partly through Wnt-induced stabilization of β-catenin. It is implicated in hepatocellular carcinoma, gastric cancer, and breast cancer progression. In fibrosis, ARHGEF19 drives excessive extracellular matrix deposition by activating hepatic stellate cells in liver fibrosis or myofibroblasts in pulmonary fibrosis. Its expression is tissue-specific, enriched in digestive organs, lungs, and during embryonic development. Recent research also links ARHGEF19 to angiogenesis and vascular permeability. Despite its pathological relevance, the precise regulatory networks and post-translational modifications remain under investigation. Targeting ARHGEF19-RhoA signaling has emerged as a therapeutic strategy, though clinical applications require further validation.
×
