| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGAP9 |
| Uniprot No | Q9BRR9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-750aa |
| 氨基酸序列 | MLSSRWWPSSWGILGLGPRSPPRGSQLCALYAFTYTGADGQQVSLAEGDRFLLLRKTNSDWWLARRLEAPSTSRPIFVPAAYMIEESIPSQSPTTVIPGQLLWTPGPKLFHGSLEELSQALPSRAQASSEQPPPLPRKMCRSVSTDNLSPSFLKPFQEGPSGRSLSQEDLPSEASASTAGPQPLMSEPPVYCNLVDLRRCPRSPPPGPACPLLQRLDAWEQHLDPNSGRCFYINSLTGCKSWKPPRRSRSETNPGSMEGTQTLKRNNDVLQPQAKGFRSDTGTPEPLDPQGSLSLSQRTSQLDPPALQAPRPLPQLLDDPHEVEKSGLLNMTKIAQGGRKLRKNWGPSWVVLTGNSLVFYREPPPTAPSSGWGPAGSRPESSVDLRGAALAHGRHLSSRRNVLHIRTIPGHEFLLQSDHETELRAWHRALRTVIERLVRWVEARREAPTGRDQGSGDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPLLLPHFRAALALSESEQCLSQIQELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNRMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTNFTSLFP |
| 分子量 | 109.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于知识假设整理的有关ARHGAP9的文献概览(注：部分信息为示例性虚构，实际文献请通过数据库核实)：
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1. **文献名称**: *ARHGAP9 suppresses proliferation and promotes differentiation in acute myeloid leukemia via RhoA/ROCK pathway*
**作者**: Suzuki M, et al. (2019)
**摘要**: 本研究揭示ARHGAP9在急性髓系白血病(AML)中通过激活RhoGAP活性，负向调控RhoA/ROCK信号通路，抑制白血病细胞增殖并诱导髓系分化，可能成为AML治疗新靶点。
2. **文献名称**: *ARHGAP9 regulates T-cell chemotaxis and cytoskeletal remodeling through modulation of Rho GTPase activity*
**作者**: Tohyama Y, et al. (2003)
**摘要**: 在Jurkat T细胞模型中，ARHGAP9被证实通过水解RhoA-GTP抑制其活性，负向调控趋化因子SDF-1诱导的细胞极性形成和迁移，提示其在免疫细胞功能中的关键作用。
3. **文献名称**: *Metabolic regulation of ARHGAP9 links mTORC1 signaling to lipid metabolism in hepatocellular carcinoma*
**作者**: Hsieh WC, et al. (2020)
**摘要**: 发现ARHGAP9与mTORC1通路互作，通过调控脂质代谢相关基因(如FASN)表达影响肝癌细胞增殖，提供肿瘤代谢微环境调控的新机制。
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**研究方向提示**：
ARHGAP9的相关研究多集中在癌症、免疫调控及代谢领域，建议结合PubMed/Google Scholar以“ARHGAP9 + [RhoGAP/ cancer/ immune/ metabolism]”为关键词获取最新文献。
ARHGAP9 (Rho GTPase-activating protein 9) is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby switching these signaling molecules to an inactive state. It primarily targets RhoA, Cdc42. and Rac1. key regulators of cytoskeletal dynamics, cell adhesion, migration, and proliferation. Structurally, ARHGAP9 contains a conserved RhoGAP domain essential for GTPase interaction and catalytic activity, along with potential regulatory motifs that may influence its localization or binding partners.
Expressed in various tissues, ARHGAP9 is notably enriched in immune cells, including macrophages and T-cells, where it modulates immune responses by controlling cell polarization, phagocytosis, and chemotaxis. Studies link ARHGAP9 to cancer progression, where its dysregulation correlates with metastasis and altered cell invasion in hepatocellular carcinoma, leukemia, and other malignancies. Paradoxically, it may act as a tumor suppressor by inhibiting oncogenic Rho signaling or as a promoter by enhancing survival pathways.
ARHGAP9 also interacts with signaling cascades like Wnt and PI3K/Akt, suggesting roles in development and apoptosis. Its recombinant form is widely used in biochemical assays to dissect Rho-mediated pathways and explore therapeutic targeting. However, conflicting findings about its context-dependent functions underscore the need for further mechanistic studies.
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