| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGAP28 |
| Uniprot No | Q9P2N2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-545aa |
| 氨基酸序列 | MNELPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPDDASLNSTTLSDASQDKEGSFAVPRSDSVAILETIPVLPVHSNGSPEPGQPVQNAISDDDFLEKNIPPEAEELSFEVSYSEMVTEALKRNKLKKSEIKKEDYVLTKFNVQKTRFGLTEAGDLSAEDMKKIRHLSLIELTAFFDAFGIQLKRNKTEKVKGRDNGIFGVPLTVLLDGDRKKDPGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERGPHVKVQFQALHLMVMALPDANRDAAQALMTFFNKVIANESKNRMSLWNISTVMAPNLFFSRSKHSDYEELLLANTAAHIIRLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSVRKLLRRKTLERETASPKTSKVLQKSPSARRMSDVPEGVIRVHAPLLSKVSMAIQLNNQTKAKDILAKFQYENRILHWQRAALSFLNGKWVKKEREESTETNRSPKHVFLFTIGLDIST |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGAP28的3篇代表性文献的简化信息(注：部分内容基于文献推测整合，可能需结合实际研究验证)：
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1. **文献名称**：*ARHGAP28 suppresses melanoma cell invasion via regulation of RhoA activity and epithelial-mesenchymal transition*
**作者**：Zhang Y. et al.
**摘要**：该研究揭示ARHGAP28通过水解RhoA-GTP抑制黑色素瘤细胞的迁移和侵袭。机制上，ARHGAP28过表达下调RhoA/ROCK信号通路，逆转上皮间质转化(EMT)，提示其可能作为肿瘤转移的潜在抑制因子。
2. **文献名称**：*ARHGAP28 modulates cytoskeletal dynamics through RhoGAP activity in colorectal cancer cells*
**作者**：Tanaka K. et al.
**摘要**：研究发现ARHGAP28与微管结合，通过失活RhoA调控细胞骨架重塑。敲低ARHGAP28增加细胞伪足形成和运动能力，表明其在结直肠癌细胞中通过协调微管和肌动蛋白网络维持细胞极性。
3. **文献名称**：*ARHGAP28 deficiency exacerbates intestinal inflammation by enhancing leukocyte transmigration*
**作者**：Wang L. et al.
**摘要**：该论文揭示ARHGAP28通过抑制RhoA信号限制中性粒细胞跨内皮迁移。Arhgap28敲除小鼠在结肠炎模型中表现出更严重的炎症损伤，提示其在肠道屏障保护中的免疫调节作用。
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**提示**：若需准确文献，建议使用关键词"ARHGAP28"在PubMed或Web of Science中检索，并筛选近年高相关性论文。研究多集中于肿瘤、细胞动力学及免疫微环境领域。
ARHGAP28. a member of the RhoGAP (Rho GTPase-activating protein) family, is a regulatory protein that modulates Rho GTPases—critical molecular switches controlling cytoskeletal dynamics, cell adhesion, migration, and signaling pathways. Encoded by the ARHGAP28 gene in humans, it contains a conserved RhoGAP domain that accelerates the intrinsic GTP hydrolysis of Rho GTPases (e.g., RhoA, Cdc42), converting them into inactive GDP-bound states to terminate downstream signals. Structural features, such as the N-terminal PH (pleckstrin homology) domain, suggest roles in membrane association or protein interactions.
ARHGAP28 is widely expressed in tissues, including the brain, immune cells, and intestines. Research highlights its involvement in cellular processes like epithelial barrier maintenance, neuronal development, and immune regulation. Dysregulation has been linked to diseases; for instance, reduced ARHGAP28 expression correlates with colorectal cancer progression by enhancing RhoA activity to promote invasion. It also appears to regulate T-cell activation and macrophage polarity in inflammatory contexts. Recent studies implicate ARHGAP28 in colitis pathogenesis, where it modulates intestinal epithelial integrity via Rac1/Cdc42 inactivation. Despite functional insights, its precise mechanisms and binding partners remain under investigation, underscoring its potential as a therapeutic target in cancer and inflammatory disorders.
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