| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGAP25 |
| Uniprot No | P42331 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPCGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMVRCREPSCFHWVLPLVQAIPCKACSRVAIWGVLGDAVAVGAAATDSSEHTLKAWPLSKSSFYWHL |
| 分子量 | 78.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人Rho鸟苷三磷酸酶活化蛋白25(ARHGAP25)的3篇示例参考文献(注：以下为模拟内容，非真实文献)：
1. **文献名称**：*ARHGAP25 regulates neutrophil migration and immune response through RhoA/ROCK signaling*
**作者**：Smith J, et al.
**摘要**：研究发现ARHGAP25通过负调控RhoA活性，抑制中性粒细胞过度迁移，在小鼠脓毒症模型中影响炎症反应的进程。
2. **文献名称**：*ARHGAP25 suppresses leukemia progression by inactivating Rac1 signaling*
**作者**：Li X, et al.
**摘要**：该文揭示ARHGAP25在急性髓系白血病(AML)中作为肿瘤抑制因子，通过降解Rac1-GTP水平抑制癌细胞增殖和侵袭。
3. **文献名称**：*ARHGAP25 deficiency exacerbates atherosclerosis via macrophage polarization dysregulation*
**作者**：Wang Y, et al.
**摘要**：研究表明，ARHGAP25缺陷通过促进巨噬细胞向促炎M1表型极化，加速动脉粥样硬化斑块形成，提示其在内膜稳态中的保护作用。
如需真实文献，建议在PubMed或Google Scholar搜索关键词“ARHGAP25”获取最新研究。
ARHGAP25 (Rho GTPase-activating protein 25) is a member of the RhoGAP family that regulates Rho GTPases, key molecular switches controlling cytoskeletal dynamics, cell migration, and signaling pathways. Encoded by the ARHGAP25 gene on chromosome 10q11.21 in humans, it contains a conserved N-terminal RhoGAP domain and a C-terminal PH domain, enabling interactions with membrane phospholipids or signaling proteins. Functionally, ARHGAP25 accelerates GTP hydrolysis in Rac1 and Cdc42 GTPases, transitioning them to an inactive GDP-bound state, thereby modulating actin remodeling, cell polarity, and motility.
Primarily expressed in immune cells, ARHGAP25 plays critical roles in leukocyte functions. In neutrophils, it regulates integrin-mediated adhesion and chemotaxis, balancing migration efficacy while preventing excessive activation during inflammation. Dysregulation of ARHGAP25 is implicated in immune disorders and cancers. For example, reduced ARHGAP25 expression in acute myeloid leukemia (AML) correlates with enhanced Rac1 activity, promoting leukemic cell proliferation and invasion. Conversely, overexpression in some solid tumors may suppress metastasis by inhibiting Rac1/Cdc42-driven pathways.
Emerging studies highlight its role in phagocytosis, immune synapse formation, and inflammatory responses in macrophages. Despite progress, mechanisms underlying ARHGAP25’s isoform-specific functions, post-translational modifications, and therapeutic potential remain under investigation. Its dual roles in cancer progression and immune regulation make it a molecule of interest for targeted therapies.
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