| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARHGAP44 |
| Uniprot No | Q17R89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-818aa |
| 氨基酸序列 | MKKQFNRMRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSHSTHKKLTACLQGQQGAEADKRSKKLPLTTLAQCLMEGSAILGDDTLLGKMLKLCGETEDKLAQELIHFELQVERDVIEPLFLLAEVEIPNIQKQRKHLAKLVLDMDSSRTRWQQTSKSSGLSSSLQPAGAKADALREEMEEAANRVEICRDQLSADMYSFVAKEIDYANYFQTLIEVQAEYHRKSLTLLQAVLPQIKAQQEAWVEKPSFGKPLEEHLTISGREIAFPIEACVTMLLECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQASNVQEQDKKLQALWNACEKLPKANHNNIRYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQAEGNITEMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNITGNYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARRPLSVATDNMMLEFYKKDGLRKIQSMGVRVMDTNWVARRGSSAGRKVSCAPPSMQPPAPPAELAAPLPSPLPEQPLDSPAAPALSPSGLGLQPGPERTSTTKSKELSPGSAQKGSPGSSQGTACAGTQPGAQPGAQPGASPSPSQPPADQSPHTLRKVSKKLAPIPPKVPFGQPGAMADQSAGQPSPVSLSPTPPSTPSPYGLSYPQGYSLASGQLSPAAAPPLASPSVFTSTLSKSRPTPKPRQRPTLPPPQPPTVNLSASSPQSTEAPMLDGMSPGESMSTDLVHFDIPSIHIELGSTLRLSPLEHMRRHSVTDKRDSEEESESTAL |
| 分子量 | 90 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARHGAP44的3篇示例参考文献(内容基于公开研究方向整理，实际文献需通过学术数据库验证)：
1. **《ARHGAP44 regulates cell migration via PH domain-mediated membrane targeting》**
- 作者：Smith A, et al.
- 摘要：揭示了ARHGAP44通过PH结构域结合细胞膜磷脂，调控RhoA活性，影响细胞骨架重组和迁移的分子机制。
2. **《ARHGAP44 acts as a tumor suppressor in acute myeloid leukemia by modulating Rho GTPase signaling》**
- 作者：Chen L, et al.
- 摘要：研究发现ARHGAP44在白血病中表达下调，通过失活RhoA/ROCK通路抑制白血病细胞增殖和侵袭，提示其抑癌作用。
3. **《A novel ARHGAP44 variant links to neurodevelopmental disorders》**
- 作者：Wang Y, et al.
- 摘要：通过全外显子测序发现ARHGAP44基因突变与神经发育异常相关，可能通过干扰神经元突触的Rho信号传导导致认知障碍。
4. **《ARHGAP44 mediates macrophage phagocytosis through spatial control of Rac1 activity》**
- 作者：Kumar R, et al.
- 摘要：阐明ARHGAP44在巨噬细胞中通过局部抑制Rac1活性，调控吞噬小体的形成和病原体清除过程。
**注意**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索确认。
ARHGAP44. a member of the RhoGAP (Rho GTPase-activating protein) family, plays a critical role in regulating Rho GTPases, which are key molecular switches controlling cytoskeletal dynamics, cell migration, and proliferation. This protein selectively enhances the intrinsic GTP hydrolysis activity of RhoA, Cdc42. and Rac1. converting them into their inactive GDP-bound states. Structurally, ARHGAP44 contains a conserved RhoGAP domain responsible for GTPase interaction and catalytic activity, along with a pleckstrin homology (PH) domain that may regulate its subcellular localization or activity.
Expression of ARHGAP44 is observed in various tissues, with particularly high levels in hematopoietic cells. Dysregulation of ARHGAP44 has been linked to pathological conditions, including acute myeloid leukemia (AML), where its overexpression correlates with disease progression. It also interacts with immune-related signaling pathways, suggesting roles in inflammation and autoimmune disorders. Recent studies highlight its involvement in cancer metastasis through modulation of cell adhesion and motility.
Recombinant human ARHGAP44. typically produced in eukaryotic expression systems like HEK293 or insect cells, retains post-translational modifications crucial for its biological functions. This engineered protein serves as a vital tool for in vitro studies deciphering Rho signaling mechanisms, high-throughput drug screening, and exploring therapeutic strategies targeting GTPase-regulated pathways. Ongoing research focuses on its dual regulatory roles in tumor suppression and oncogenesis, depending on cellular context.
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